Growth arrest and DNA damage inducible 45-beta activates pro-inflammatory cytokines and phagocytosis in the grass carp (Ctenopharyngodon idella) after Aeromonas hydrophila infection

• Published in: Developmental and comparative immunology 2018-10, Vol.87, p.176-181
• Main Authors: Bai, Yulin, Shen, Yubang, Xu, Xiao-yan, Bai, Yuqi, Fang, Yuan, Zhang, Meng, Miao, Yiheng, Zhang, Xueshu, Li, Jia-le
• Format: Article
• Language: English
• Subjects: Apoptosis; Blood cells; Carp; Ctenopharyngodon idella; Cytokines; Demethylation; Deoxyribonucleic acid; DNA; DNA damage; Gadd45B; Grass carp; Immune response; Inflammation; Innate immune response; Innate immunity; Interleukin 8; Kinases; MAP kinase; Phagocytosis; Proteins; Tumor necrosis factor-α
• ISSN: 0145-305X; 1879-0089
• DOI: 10.1016/j.dci.2018.06.010

Growth arrest and DNA damage inducible 45-beta (Gadd45B) is essential for mitogen-activated protein kinases (MAPK) activities, and involved in regulating growth, apoptosis, and DNA demethylation. In the present study, the cDNA of gcGadd45Ba and gcGadd45Bb in grass carp was identified. And the expression levels show that they were widely distributed in the tested tissues and showed significant immune responses both in vitro and in vivo after challenge with bacteria and pathogen-associated molecular patterns (PAMPs). Overexpression of Gadd45B significantly induced the expression of pro-inflammatory cytokines (IL-1β, IL-8, and TNF-α) and enhanced the phagocytosis activation of grass carp blood cells. These results indicate that Gadd45B plays an important role in innate immune responses. •We cloned two Gadd45B gene subtypes (gcGadd45Ba and gcGadd45Bb) in grass carp.•gcGadd45Ba and gcGadd45Bb were expressed in all the tested tissues of grass carp.•gcGadd45Ba and gcGadd45Bb promoted pathogen phagocytosis by grass carp blood cells.•Overexpression of gcGadd45Ba and gcGadd45Bb activated immune genes to resist pathogen infection.•gcGadd45Ba and gcGadd45Bb play important roles in the innate immunity of grass carp.