Phenotypic and molecular characterization of Staphylococcus aureus strains of veterinary, dairy and human origin

• Published in: Epidemiology and infection 2009-05, Vol.137 (5), p.688-699
• Main Authors: GONANO, M., HEIN, I., ZANGERL, P., RAMMELMAYR, A., WAGNER, M.
• Format: Article
• Language: English
• Subjects: AFLP; Amplified Fragment Length Polymorphism Analysis; Animals; Anti-Bacterial Agents - pharmacology; Antibiotic resistance; Antimicrobial agents; Antimicrobials; Bacterial Proteins - metabolism; Bacterial Typing Techniques; Bacteriology; Biological and medical sciences; Cluster Analysis; Coagulase - metabolism; DNA Fingerprinting; DNA, Bacterial - genetics; Enterotoxins; Enterotoxins - genetics; Enterotoxins - metabolism; Food Microbiology; Fundamental and applied biological sciences. Psychology; Genotype; genotyping; Humans; Lipase - metabolism; Microbial Sensitivity Tests; Microbiology; Micrococcal Nuclease - metabolism; Miscellaneous; Molecular Epidemiology; Polymerase chain reaction; Polymorphism; Software; Staphylococcal Infections - microbiology; Staphylococcal Infections - veterinary; Staphylococcus; Staphylococcus aureus; Staphylococcus aureus - classification; Staphylococcus aureus - genetics; Staphylococcus aureus - isolation & purification; Staphylococcus aureus - physiology; Toxins; Ungulates; Veterinary medicine
• ISSN: 0950-2688; 1469-4409
• DOI: 10.1017/S0950268808001453

Austrian veterinary (n=91), dairy (n=86), and human strains (n=48) of Staphylococcus aureus were tested for various phenotypic properties including clumping factor, egg-yolk reaction, production of thermonuclease and susceptibility to 14 antibiotics. In addition the expression of enterotoxins (A–E), and the presence of enterotoxin genes sea to sej and tst was determined. Significant differences in antimicrobial susceptibility were found with 84·6% of veterinary, 57·0% of dairy, and 20·8% of human strains susceptible to all antibiotics tested (P