Cultivable bacteria from bulk water, aggregates, and surface sediments of a tidal flat ecosystem

Most-probable-number (MPN) dilution series were used to enumerate and isolate bacteria from bulk water, suspended aggregates, the oxic layer, and the oxic-anoxic transition zone of the sediment of a tidal flat ecosystem in the southern North Sea. The heterotrophic aerobic bacteria were able to grow...

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Bibliographic Details
Published in:Ocean dynamics 2009-04, Vol.59 (2), p.291-304
Main Authors: Stevens, Heike, Simon, Meinhard, Brinkhoff, Thorsten
Format: Article
Language:English
Subjects:
Actinobacteria
Aerobic bacteria
Aggregates
Atmospheric Sciences
Bacteria
biopolymers
Cellulose
Chitin
Earth and Environmental Science
Earth Sciences
Flavobacteria
Fluid- and Aerodynamics
Fucus vesiculosus
Geophysics/Geodesy
Heterotrophic bacteria
Marine
Microbiology
Monitoring/Environmental Analysis
MPN cultures
Oceanography
Sediments
Substrates
Tidal flats
Transition zone
Wadden sea
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Summary:Most-probable-number (MPN) dilution series were used to enumerate and isolate bacteria from bulk water, suspended aggregates, the oxic layer, and the oxic-anoxic transition zone of the sediment of a tidal flat ecosystem in the southern North Sea. The heterotrophic aerobic bacteria were able to grow on agar-agar, alginate, cellulose, chitin, dried and ground Fucus vesiculosus, Marine Broth 2216, palmitate, and starch. MPN counts of bulk water and aggregate samples ranged between 0.18 x 10¹ and 1.1 x 10⁶ cells per milliliter and those of the sediment surface and the transition zone between 0.8 x 10¹ and 5.1 x 10⁷ cells per gram dry weight. Marine Broth and F. vesiculosus yielded the highest values of all substrates tested and corresponded to 2.3-32% of 4,6-diamidinophenyl indole cell counts. Strains of seven phylogenetic classes were obtained: Actinobacteria, Bacilli, α- and γ-Proteobacteria, Sphingobacteria, Flavobacteria, and Planctomycetacia. Only with agar-agar as substrate could organisms of all seven classes be isolated.
ISSN:1616-7341
1616-7228
DOI:10.1007/s10236-008-0168-z