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Dual role of NER in mutagenesis in Pseudomonas putida
Nucleotide excision repair (NER) is one of the most important repair systems which counteracts different forms of DNA damage either induced by various chemicals or irradiation. At the same time, less is known about the functions of NER in repair of DNA that is not exposed to exogenous DNA-damaging a...
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Published in: | DNA repair 2008, Vol.7 (1), p.20-30 |
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Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Nucleotide excision repair (NER) is one of the most important repair systems which counteracts different forms of DNA damage either induced by various chemicals or irradiation. At the same time, less is known about the functions of NER in repair of DNA that is not exposed to exogenous DNA-damaging agents. We have investigated the role of NER in mutagenesis in
Pseudomonas putida. The genome of this organism contains two
uvrA genes,
uvrA and
uvrA2. Genetic studies on the effects of
uvrA,
uvrA2,
uvrB and
UvrC in mutagenic processes revealed that all of these genes are responsible for the repair of UV-induced DNA damage in
P. putida. However,
uvrA plays more important role in this process than
uvrA2 since the deletion of
uvrA2 gene had an effect on the UV-tolerance of bacteria only in the case when
uvrA was also inactivated. Interestingly, the lack of functional
uvrB,
uvrC or
uvrA2 gene reduced the frequency of stationary-phase mutations. The contribution of
uvrA2,
uvrB and
uvrC to the mutagenesis appeared to be most significant in the case of 1-bp deletions whose emergence is dependent on error-prone DNA polymerase Pol IV. These data imply that NER has a dual role in mutagenesis in
P. putida—besides functioning in repair of damaged DNA, NER is also important in generation of mutations. We hypothesize that NER enzymes may initiate gratuitous DNA repair and the following DNA repair synthesis might be mutagenic. |
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ISSN: | 1568-7864 1568-7856 |
DOI: | 10.1016/j.dnarep.2007.07.008 |