Short communication: Extended-spectrum AmpC–producing Escherichia coli from milk and feces in dairy farms in Brazil

The AmpC enzyme is normally expressed constitutively in Escherichia coli, and its overproduction confers resistance to cefoxitin. A newly reported AmpC, the extended-spectrum AmpC (ESAC), is related to resistance to cefepime, a fourth-generation cephalosporin. This enzyme presents more flexibility i...

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Bibliographic Details
Published in:Journal of dairy science 2018-09, Vol.101 (9), p.7808-7811
Main Authors: Santiago, G.S., Coelho, I.S., Bronzato, G.F., Moreira, A.B., Gonçalves, D., Alencar, T.A., Ferreira, H.N., Castro, B.G., Souza, M.M.S., Coelho, S.M.O.
Format: Article
Language:English
Subjects:
AmpC
Animals
Bacterial Proteins
beta-Lactamases
Brazil
Cattle
cefepime
chromosomal gene
Drug Resistance, Bacterial
Escherichia coli - enzymology
Escherichia coli - isolation & purification
Escherichia coli Infections
Farms
Feces - microbiology
Humans
Microbial Sensitivity Tests
Milk - microbiology
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Summary:The AmpC enzyme is normally expressed constitutively in Escherichia coli, and its overproduction confers resistance to cefoxitin. A newly reported AmpC, the extended-spectrum AmpC (ESAC), is related to resistance to cefepime, a fourth-generation cephalosporin. This enzyme presents more flexibility in the active site due to insertions, replacements, and deletions on AA sequences. Many isolates producing ESAC were reported in human clinical isolates, but E. coli ESAC producers were reported in animals only in France. The animal E. coli strains can produce this enzyme and possibly disseminate it to human and production environments. In our study, 3 strains of E. coli from milk and feces bovine samples, collected in Rio de Janeiro, Brazil, were suspected to produce ESAC. After excluding other mechanisms of resistance, the gene was sequenced to verify ESAC characteristics. These strains presented replacement of AA in omega and R2 loops, suggesting ESAC production. This is the first report to study ESAC E. coli in dairy farms in Brazil.
ISSN:0022-0302
1525-3198
DOI:10.3168/jds.2017-13658