Systems-Based Interactome Analysis for Hematopoiesis Effect of Angelicae sinensis Radix: Regulated Network of Cell Proliferation towards Hemopoiesis

Objective To explore the molecular-level mechanism on the hematopoiesis effect of Angelicae sinensis Radix (ASR) with systems-based interactome analysis. Methods This systems-based interactome analysis was designed to enforce the workflow of "ASR (herb)→compound→target protein→internal protein...

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Bibliographic Details
Published in:Chinese journal of integrative medicine 2019-12, Vol.25 (12), p.939-947
Main Authors: Zheng, Guang, Zhang, He, Yang, Yun, Sun, Ying-li, Zhang, Yan-jing, Chen, Ju-ping, Hao, Ting, Lu, Cheng, Guo, Hong-tao, Zhang, Ge, Fan, Dan-ping, He, Xiao-juan, Lu, Ai-ping
Format: Article
Language:English
Subjects:
Angelica sinensis - chemistry
Animals
Bone Marrow - drug effects
Cell Proliferation - drug effects
Drugs, Chinese Herbal - pharmacology
Hematopoiesis - drug effects
Interdisciplinary Knowledge
Janus Kinase 2 - metabolism
Medicine
Medicine & Public Health
Mice
Mice, Inbred BALB C
Plant Roots - chemistry
Protein-Tyrosine Kinases - metabolism
Syk Kinase - metabolism
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Summary:Objective To explore the molecular-level mechanism on the hematopoiesis effect of Angelicae sinensis Radix (ASR) with systems-based interactome analysis. Methods This systems-based interactome analysis was designed to enforce the workflow of "ASR (herb)→compound→target protein→internal protein actions→ending regulated protein for hematopoiesis". This workflow was deployed with restrictions on regulated proteins expresses in bone marrow and anemia disease and futher validated with experiments. Results The hematopoiesis mechanism of ASR might be accomplished through regulating pathways of cell proliferation towards hemopoiesis with cross-talking agents of spleen tyrosine kinase (SYK), Janus kinase 2 (JAK2), and interleukin-2-inducible T-cell kinase (ITK). The hematopoietic function of ASR was also validated by colony-forming assay performed on mice bone marrow cells. As a result, SYK, JAK2 and ITK were activated. Conclusion This study provides a new approach to systematically study and predict the therapeutic mechanism for ASR based on interactome analysis towards biological process with experimental validations.
ISSN:1672-0415
1993-0402
DOI:10.1007/s11655-018-3003-5