Somatic embryo proliferation, maturation and germination in Catharanthus roseus

In the present study an efficient somatic embryogenesis method has been developed in Catharanthus roseus. Friable embryogenic callus was induced from hypocotyl of in vitro germinated seeds on Murashige and Skoog basal nutrient media supplemented with various auxins particularly 2,4-D (1.0 mg l-1). H...

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Published in:Plant cell, tissue and organ culture tissue and organ culture, 2006-03, Vol.84 (3), p.325-332
Main Authors: Junaid, A, Mujib, A, Bhat, M.A, Sharma, M.P
Format: Article
Language:English
Subjects:
auxins
benzyladenine
Biological and medical sciences
Biotechnology
callus
Catharanthus roseus
culture media
dose response
Eukaryotic cell cultures
explants
fructose
Fundamental and applied biological sciences. Psychology
gibberellic acid
hypocotyls
in vitro culture
in vitro regeneration
maltose
methodology
Methods. Procedures. Technologies
micropropagation
Miscellaneous
naphthaleneacetic acid
Plant cells and fungal cells
seed germination
somatic embryogenesis
somatic embryos
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Summary:In the present study an efficient somatic embryogenesis method has been developed in Catharanthus roseus. Friable embryogenic callus was induced from hypocotyl of in vitro germinated seeds on Murashige and Skoog basal nutrient media supplemented with various auxins particularly 2,4-D (1.0 mg l-1). However, only NAA (1.0 mg l-1) produced somatic embryos in cultures. Embryo proliferation was even high on the same medium added with BAP. Cotyledonary somatic embryo germinated and converted into plantlets in BAP (0.5 mg l-1) added medium following a treatment with gibberellic acid (1.0 mg l-1) for maturation. Carbon sources and concentrations had a marked influence on maturation process. Plantlet conversion was better achieved when embryos were matured on 3% fructose or 3-6% maltose. The result discussed in this paper indicates that somatic embryos were produced in numbers and converted plantlets can be used as raw material, genetic modification to embryo precursor cell may improve alkaloid yield further.
ISSN:0167-6857
1573-5044
DOI:10.1007/s11240-005-9041-7