Bone Targeted Delivery of SDF‑1 via Alendronate Functionalized Nanoparticles in Guiding Stem Cell Migration

• Published in: ACS applied materials & interfaces 2018-07, Vol.10 (28), p.23700-23710
• Main Authors: Chen, Qingchang, Zheng, Chuping, Li, Yanqun, Bian, Shaoquan, Pan, Haobo, Zhao, Xiaoli, Lu, William W
• Format: Article
• Language: English
• Subjects: Alendronate; Animals; Bone and Bones; Cell Movement; Chemokine CXCL12; Mesenchymal Stem Cells; Mice; Nanoparticles; Stem Cells
• ISSN: 1944-8244; 1944-8252
• DOI: 10.1021/acsami.8b08606

Stem cells are well-known for their great capacity for tissue regeneration. This provides a promising source for cell-based therapies in treating various bone degenerative disorders. However, the major hurdles for their application in transplantation are the poor bone marrow homing and engraftment efficiencies. Stromal cell-derived factor 1 (SDF-1) has been identified as a major stem cell homing factor. With the aims of bone targeted SDF-1 delivery and regulating MSCs migration, alendronate modified liposomal nanoparticles (Aln-Lipo) carrying SDF-1 gene were developed in this study. Alendronate modification significantly increased the mineral binding affinity of liposomes, and facilitated the gene delivery to osteoblastic cells. Up-regulated SDF-1 expression in osteoblasts triggered MSCs migration. Systemic infusion of Aln-Lipo-SDF-1 with fluorescence labeling in mice showed the accumulation in osseous tissue by biophotonic imaging. Corresponding to the delivered SDF-1, the transplanted GFP+ MSCs were attracted to bone marrow and contributed to bone regeneration. This study may provide a useful technique in regulating stem cell migration.