Comparative genetic diversity of Triticum aestivum-Triticum polonicum introgression lines with long glume and Triticum petropavlovskyi by AFLP-based assessment

• Published in: Genetic resources and crop evolution 2008-02, Vol.55 (1), p.133-141
• Main Authors: Akond, A. S. M. G. Masum, Watanabe, N, Furuta, Y
• Format: Article
• Language: English
• Subjects: Agriculture; Amplified fragment length polymorphism; Biomedical and Life Sciences; Breeding methods; Bulk breeding method; Gene polymorphism; Genetic diversity; Genomes; introgression; Life Sciences; Loci; Long glume; Phenotypes; Plant Genetics and Genomics; Plant Physiology; Plant Sciences; Plant Systematics/Taxonomy/Biogeography; Polymorphism; Research Article; Triticum; Triticum aestivum; Triticum petropavlovskyi; Triticum polonicum; Wheat
• ISSN: 0925-9864; 1573-5109
• DOI: 10.1007/s10722-007-9221-x

Genetic diversity of a set of introgression lines of Triticum aestivum L./T. polonicum L. with long glume and T. petropavlovskyi Udacz. et Migusch. were analyzed by Amplified Fragments Length Polymorphism (AFLP). Small-scale bulk breeding method was applied throughout until F₆ generation to develop the introgression lines. Thirty-eight hexapolid F₇ plants with long glume phenotype and their parents were subjected to AFLP analysis by four primer combinations. A total of 47 polymorphic loci were detected between the parents, 15 of them were introgressed across the 38 lines. It was hypothesized that approximately 50% of A or B genomes associated polymorphic loci were introgressed. The variation of introgression lines was limited within the diversity between their parents, T. aestivum L. cv. Novosibirskaya 67 (N67) and T. polonicum L. cv. IC12196. N67 was closer to 38 introgression lines than that of IC12196. The UPGMA cluster and principal coordinate analysis (PCO) grouping showed 0.84 to 0.98 similarity values between N67 and the introgression lines. Eleven T. petropavlovskyi accessions were distinguished from introgression lines with UPGMA clusters and PCO groupings, and T. petropavlovskyi was located between the introgressions lines and IC12196. Several introgression lines resembled with T. petropavlovskyi for awning and glume length. The genetic variation among 38 introgression lines was much wider than that of T. petropavlovskyi. We concluded that T. petropavlovskyi was established by intensive selection of hybrid between T. aestivum/T. polonicum.