Detection of Soft Wheat in Semolina and Durum Wheat Bread by Analysis of DNA Microsatellites

The aim of this work was to evaluate the analysis of DNA microsatellites for the detection of soft wheat (Triticum aestivum L.) in semolina and durum wheat bread (prepared from Triticum turgidum L. var. durum). The results enabled selection of an efficient D-genome-specific repetitive DNA sequence t...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2007-05, Vol.55 (9), p.3312-3318
Main Authors: Pasqualone, Antonella, Montemurro, Cinzia, Grinn-Gofron, Agnieszka, Sonnante, Gabriella, Blanco, Antonio
Format: Article
Language:English
Subjects:
adulterated products
Biological and medical sciences
Bread - analysis
breads
Cereal and baking product industries
DNA, Plant - analysis
durum wheat
Flour - analysis
Food Contamination - analysis
Food industries
Fundamental and applied biological sciences. Psychology
General aspects
genetic markers
Methods of analysis, processing and quality control, regulation, standards
microsatellite repeats
Microsatellite Repeats - genetics
Polymerase Chain Reaction
product authenticity
semolina
soft white wheat
Triticum - chemistry
Triticum - genetics
Triticum aestivum
Triticum turgidum
Triticum turgidum subsp. durum
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Summary:The aim of this work was to evaluate the analysis of DNA microsatellites for the detection of soft wheat (Triticum aestivum L.) in semolina and durum wheat bread (prepared from Triticum turgidum L. var. durum). The results enabled selection of an efficient D-genome-specific repetitive DNA sequence to detect common wheat in semolina and breads by qualitative PCR with a threshold of 3 and 5%, respectively, lowered to 2.5% by real-time PCR. This is of major importance for checking during production of some typical products recently awarded the European Protected Designation of Origin (PDO) mark such as Altamura bread, which should not contain soft wheat flour. The feasibility of quantification of common wheat adulteration in semolina using real-time PCR was also demonstrated. Keywords: Durum wheat; bread; DNA microsatellites; soft wheat detection; polymerase chain reaction
ISSN:0021-8561
1520-5118
DOI:10.1021/jf063383e