Activation of immunological responses in Litopenaeus setiferus hemocytes by a hemocyanin like-lectin

In order to identify the specific role of humoral lectins in crustaceans' immune response, we evaluated the effect of the 291 kDa hemocyanin-like lectin from Litopenaeus setiferus (LsL) on its own hemocytes. LsL binds 5.6% of circulating non-activated hemocytes, but is able to recognize 85% of...

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Bibliographic Details
Published in:Aquaculture 2009-07, Vol.292 (1), p.11-15
Main Authors: Alpuche, Juan, Rosas, Carlos, Vázquez, Lorena, Guevara, Jorge, Pereyra, Ali, Agundis, Concepción, Pascual, Cristina, Zenteno, Edgar
Format: Article
Language:English
Subjects:
Aquaculture
Crustacean
Crustaceans
Glycoproteins
Hemocyanin
Immunology
Lectin
Litopenaeus setiferus
Marine
Membranes
N-acetylated carbohydrates
Oxidation
Oxidative burst
Reactive oxygen intermediates
Receptor
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Summary:In order to identify the specific role of humoral lectins in crustaceans' immune response, we evaluated the effect of the 291 kDa hemocyanin-like lectin from Litopenaeus setiferus (LsL) on its own hemocytes. LsL binds 5.6% of circulating non-activated hemocytes, but is able to recognize 85% of total adhered (activated) hemocytes, showing filipodia and pseudopodia, inducing the production of reactive oxygen intermediates (ROIs) in a time- and dose-dependent manner (100 ng/10 5 cells/20 min). This effect was inhibited specifically by N-acetylated sugars (NeuAc, GalNAc or GlcNAc), as well as by superoxide dismutase (SOD), acetylsalicylic, and mefenamic acid. In hemocytes, LsL also induced higher ROIs production than Zymozan ( p > 0.05). The receptor for LsL in the hemocytes membrane is a glycoprotein of 61 and 52 kDa per subunit, as determined by western blot. Our results suggest that LsL participates in the activation of oxidative immune responses of granular hemocytes induced by NADPH and other oxidative pathways through a specific membrane receptor.
ISSN:0044-8486
1873-5622
DOI:10.1016/j.aquaculture.2009.03.022