Electron microscopic study of Burkholderia cepacia biofilms

Using the methods of transmission electron microscopy, the structure of the biofilms formed by the bacterium Burkholderia cepacia (clinical isolate and mutants with an increased and decreased ability to produce biofilm) were investigated. The biofilms were obtained on a liquid nutrient medium or on...

Full description

Saved in:
Bibliographic Details
Published in:Microbiology (New York) 2008-02, Vol.77 (1), p.55-61
Main Authors: Smirnova, T A, Didenko, LV, Andreev, AL, Alekseeva, N V, Stepanova, T V, Romanova, YuM
Format: Article
Language:English
Subjects:
Burkholderia cepacia
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Using the methods of transmission electron microscopy, the structure of the biofilms formed by the bacterium Burkholderia cepacia (clinical isolate and mutants with an increased and decreased ability to produce biofilm) were investigated. The biofilms were obtained on a liquid nutrient medium or on an abiotic surface (polystyrene). It has been demonstrated that the cultures of the studied strains differ in some morphological and functional characteristics. In biofilms, changes in the size and submicroscopic organization of all the components of bacterial cells occur. Staining biofilms with ruthenium red revealed the presence of exopolysaccharides in the intercellular space. The differences in the ultrastructure of bacterial films formed on nutrient medium and abiotic surfaces were demonstrated.
ISSN:0026-2617
1608-3237
DOI:10.1007/s11021-008-1008-3