Training state and fasting-induced PDH regulation in human skeletal muscle

The aim of the present study was to examine the influence of training state on fasting-induced skeletal muscle pyruvate dehydrogenase (PDH) regulation, including PDH phosphorylation. Trained and untrained subjects, matched for skeletal muscle CS activity and OXPHOS protein, fasted for 36 h after rec...

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Published in:Pflügers Archiv 2018-11, Vol.470 (11), p.1633-1645
Main Authors: Gudiksen, Anders, Bertholdt, Lærke, Stankiewicz, Tomasz, Villesen, Ida, Bangsbo, Jens, Plomgaard, Peter, Pilegaard, Henriette
Format: Article
Language:English
Subjects:
Acetylation
Adult
Biomedical and Life Sciences
Biomedicine
Cell Biology
Exercise
Fasting
Fasting - metabolism
Human Physiology
Humans
Molecular Medicine
Muscle Physiology
Muscle, Skeletal - metabolism
Muscle, Skeletal - physiology
Musculoskeletal system
Neurosciences
Oxidation
Oxygen Consumption
Phosphorylation
Proteins
Pyruvate Dehydrogenase Complex - metabolism
Pyruvic acid
Receptors
Skeletal muscle
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Summary:The aim of the present study was to examine the influence of training state on fasting-induced skeletal muscle pyruvate dehydrogenase (PDH) regulation, including PDH phosphorylation. Trained and untrained subjects, matched for skeletal muscle CS activity and OXPHOS protein, fasted for 36 h after receiving a standardized meal. Respiratory exchange ratio (RER) was measured and blood as well as vastus lateralis muscle biopsies were obtained 2, 12, 24, and 36 h after the meal. RER decreased with fasting only in untrained individuals, while PDHa activity decreased from 12 h after the meal in untrained, but only tended to decrease at 36 h in trained. PDH-E1α, PDP1 protein, PDH phosphorylation, and PDH acetylation in skeletal muscle was higher in trained than untrained subjects, but did not change with fasting, while PDK4 protein was higher at 36 h than at 2 h after the meal in both groups. In conclusion, the present results suggest that endurance exercise training modifies the fasting-induced regulation of PDHa activity in skeletal muscle and the substrate switch towards fat oxidation. PDH phosphorylation could not explain the fasting-induced regulation of PDHa activity suggesting other post translational modifications.
ISSN:0031-6768
1432-2013
DOI:10.1007/s00424-018-2164-6