Strain Construction for Ethanol Production from Dilute-Acid Lignocellulosic Hydrolysate

In order to construct a strain that converts sugar mixture and resist/metabolize inhibitors in lignocellulosic dilute-acid hydrolysate, the biotechnology of inactive intergeneric fusion between Saccharomyces cerevisiae and Pachysolen tannophilis was performed. Fusant 1 was successfully obtained as a...

Full description

Saved in:
Bibliographic Details
Published in:Applied biochemistry and biotechnology 2009-06, Vol.157 (3), p.473-482
Main Authors: Yan, Fei, Bai, Fali, Tian, Shen, Zhang, Jinxin, Zhang, Zuoyang, Yang, Xiushan
Format: Article
Language:English
Subjects:
Biochemistry
Biological and medical sciences
Biotechnology
Cellulose
Chemistry
Chemistry and Materials Science
Ethanol
Ethanol - metabolism
ethanol production
Fermentation
Fundamental and applied biological sciences. Psychology
genetic engineering
glucose
hydrolysates
Industrial Microbiology - methods
Lignin - metabolism
lignocellulosic wastes
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
Pachysolen tannophilus
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
strains
Sugar
xylitol
Xylitol - metabolism
xylose
Yeasts - genetics
Yeasts - metabolism
yields
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In order to construct a strain that converts sugar mixture and resist/metabolize inhibitors in lignocellulosic dilute-acid hydrolysate, the biotechnology of inactive intergeneric fusion between Saccharomyces cerevisiae and Pachysolen tannophilis was performed. Fusant 1 was successfully obtained as a hybrid strain, which was screened out by xylose and mixed sugar (xylose and glucose) fermentation. This strain showed good abilities of ethanol production, ethanol tolerance, and resistance to the toxic inhibitors presenting in the hydrolysate. The maximum volumetric yield of ethanol and yield of xylitol in mixed sugar was 9.52 g/l and 0.44 g/g, respectively. The results indicated that the constructed strain Fusant 1 was a good producer for ethanol and xylitol from lignocellulosic dilute-acid hydrolysate.
ISSN:0273-2289
1559-0291
DOI:10.1007/s12010-008-8343-8