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Strain Construction for Ethanol Production from Dilute-Acid Lignocellulosic Hydrolysate

In order to construct a strain that converts sugar mixture and resist/metabolize inhibitors in lignocellulosic dilute-acid hydrolysate, the biotechnology of inactive intergeneric fusion between Saccharomyces cerevisiae and Pachysolen tannophilis was performed. Fusant 1 was successfully obtained as a...

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Published in:	Applied biochemistry and biotechnology 2009-06, Vol.157 (3), p.473-482
Main Authors:	Yan, Fei, Bai, Fali, Tian, Shen, Zhang, Jinxin, Zhang, Zuoyang, Yang, Xiushan
Format:	Article
Language:	English
Subjects:	Biochemistry Biological and medical sciences Biotechnology Cellulose Chemistry Chemistry and Materials Science Ethanol Ethanol - metabolism ethanol production Fermentation Fundamental and applied biological sciences. Psychology genetic engineering glucose hydrolysates Industrial Microbiology - methods Lignin - metabolism lignocellulosic wastes Methods. Procedures. Technologies Microbial engineering. Fermentation and microbial culture technology Pachysolen tannophilus Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism strains Sugar xylitol Xylitol - metabolism xylose Yeasts - genetics Yeasts - metabolism yields
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creator	Yan, Fei Bai, Fali Tian, Shen Zhang, Jinxin Zhang, Zuoyang Yang, Xiushan
description	In order to construct a strain that converts sugar mixture and resist/metabolize inhibitors in lignocellulosic dilute-acid hydrolysate, the biotechnology of inactive intergeneric fusion between Saccharomyces cerevisiae and Pachysolen tannophilis was performed. Fusant 1 was successfully obtained as a hybrid strain, which was screened out by xylose and mixed sugar (xylose and glucose) fermentation. This strain showed good abilities of ethanol production, ethanol tolerance, and resistance to the toxic inhibitors presenting in the hydrolysate. The maximum volumetric yield of ethanol and yield of xylitol in mixed sugar was 9.52 g/l and 0.44 g/g, respectively. The results indicated that the constructed strain Fusant 1 was a good producer for ethanol and xylitol from lignocellulosic dilute-acid hydrolysate.
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Fusant 1 was successfully obtained as a hybrid strain, which was screened out by xylose and mixed sugar (xylose and glucose) fermentation. This strain showed good abilities of ethanol production, ethanol tolerance, and resistance to the toxic inhibitors presenting in the hydrolysate. The maximum volumetric yield of ethanol and yield of xylitol in mixed sugar was 9.52 g/l and 0.44 g/g, respectively. The results indicated that the constructed strain Fusant 1 was a good producer for ethanol and xylitol from lignocellulosic dilute-acid hydrolysate.</description><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cellulose</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Ethanol</subject><subject>Ethanol - metabolism</subject><subject>ethanol production</subject><subject>Fermentation</subject><subject>Fundamental and applied biological sciences. 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subjects	Biochemistry Biological and medical sciences Biotechnology Cellulose Chemistry Chemistry and Materials Science Ethanol Ethanol - metabolism ethanol production Fermentation Fundamental and applied biological sciences. Psychology genetic engineering glucose hydrolysates Industrial Microbiology - methods Lignin - metabolism lignocellulosic wastes Methods. Procedures. Technologies Microbial engineering. Fermentation and microbial culture technology Pachysolen tannophilus Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism strains Sugar xylitol Xylitol - metabolism xylose Yeasts - genetics Yeasts - metabolism yields
title	Strain Construction for Ethanol Production from Dilute-Acid Lignocellulosic Hydrolysate
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