X-ray absorption spectroscopy investigation of speciation and biotransformation of copper in Elsholtzia splendens

• Published in: Plant and soil 2008, Vol.302 (1-2), p.163-174
• Main Authors: Shi, Jiyan, Wu, Bei, Yuan, Xiaofeng, YY, Cao, Chen, Xincai, Chen, Yingxu, Hu, Tiandou
• Format: Article
• Language: English
• Subjects: Absorption spectroscopy; Agronomy. Soil science and plant productions; Animal, plant and microbial ecology; Biological and medical sciences; Biomedical and Life Sciences; Biotransformation; Cell wall; Cell walls; Cells; Copper; Detoxification; Ecology; Elsholtzia; Elsholtzia splendens; Fundamental and applied biological sciences. Psychology; Leaves; Life Sciences; Ligands; Metallothioneins; Phytotoxicity; Plant biology; Plant growth; Plant Physiology; Plant roots; Plant Sciences; Plants; Regular Article; Roots; Soil Science & Conservation; Soils; Speciation; Spectrum analysis; Stems; Vacuoles; X-ray absorption spectroscopy
• ISSN: 0032-079X; 1573-5036
• DOI: 10.1007/s11104-007-9463-6

Elsholtzia splendens is a Cu-tolerant plant growing in copper mine areas in the south of China. In this study, X-ray absorption spectroscopy (XAS) was used to investigate the Cu speciation and biotransformation in E. splendens with 300 μM Cu treatment from 10 days to 60 days. The results showed that 300 μM Cu was phytotoxic to E. spendens. The Cu K-edge X-ray absorption near edge structure (XANES) revealed that most copper in roots, stems and leaves exists as divalent Cu. Cu speciation changed depending on the treatment time, but there was no unidirectional trend in roots, stems, and leaves. The percentages of potential Cu ligands in all samples were estimated by fitting the XANES spectra with linear combinations. Most Cu in roots, stems and leaves was bound with cell wall and histidine (His)-like ligands, while a minor proportion of the Cu was bound to oxalate and glutathione-like ligands. The fitting results of Cu K-edge extended X-ray absorption fine structure (EAXFS) showed that nitrogen/oxygen (N/O) ligands were dominant in roots, stems and leaves of the plant, while S ligands were rare. All these results suggest that Cu bound by N/O ligands plays a key role in Cu detoxification of E. splendens, and a role for classical metal-detoxifying S ligands, such as metallothioneins and phytochelatins, in Cu detoxification of E. splendens is not supported in the present study. Due to the phytotoxicity of 300 μM Cu to E. splendens, the question of whether S ligands play a significant role in Cu detoxification in E. splendens exposed to lower levels of Cu should be further studied.