Expression profile of human tissue kallikrein 15 provides preliminary insights into its roles in the prostate and testis

Human tissue kallikrein 15 (KLK15) is the latest member of the kallikrein-related peptidase family. Little is known about the pathophysiological roles of KLK15. Previous studies implied a role of KLK15 in prostate cancer. In the present study, we examined KLK15 protein expression using a new immunoa...

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Bibliographic Details
Published in:Clinical biochemistry 2018-09, Vol.59, p.78-85
Main Authors: Filippou, Panagiota S., Ren, Annie H., Soosaipillai, Antoninus, Papaioannou, Michail-Dimitrios, Korbakis, Dimitrios, Safar, Roaa, Diamandis, Eleftherios P., Conner, James R.
Format: Article
Language:English
Subjects:
Adult
Cancer cell lines
Cell Line, Tumor
Enzyme-Linked Immunosorbent Assay
Humans
Immunoassays
Immunohistochemistry
Jurkat Cells
Kallikreins
Kallikreins - biosynthesis
Kallikreins - genetics
Kallikreins - metabolism
KLK15
Male
Prostate - enzymology
Prostate cancer
Prostatic Neoplasms - enzymology
RNA, Messenger - genetics
RNA, Messenger - metabolism
Semen - enzymology
Testis - enzymology
Transcriptome
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Summary:Human tissue kallikrein 15 (KLK15) is the latest member of the kallikrein-related peptidase family. Little is known about the pathophysiological roles of KLK15. Previous studies implied a role of KLK15 in prostate cancer. In the present study, we examined KLK15 protein expression using a new immunoassay (ELISA) and immunohistochemistry (IHC). Highest KLK15 levels were detected in the testis and seminal fluid, whereas lower levels were observed in prostate and other tissues. Immunohistochemical analysis of testis suggests that KLK15 is strongly expressed in mature spermatids, but not in immature germ cells. KLK15 displayed predominantly nuclear localization in the basal cell layer of the prostatic epithelium. We also measured KLK15 in supernatants of various cell lines. Highest KLK15 levels were primarily detected in prostate cancer cell lines and KLK15 expression was hormone-independent, in contrast to KLK3. Collectively, our data provide insights into the localization and possible role of KLK15 in human physiology.
ISSN:0009-9120
1873-2933
DOI:10.1016/j.clinbiochem.2018.06.017