Induction of Coronary Arteritis with Administration of CAWS (Candida albicans Water-Soluble Fraction) Depending on Mouse Strains

• Published in: Immunopharmacology and immunotoxicology 2004, Vol.26 (4), p.527-543
• Main Authors: Nagi-Miura, Noriko, Shingo, Yuko, Adachi, Yoshiyuki, Ishida-Okawara, Akiko, Oharaseki, Toshiaki, Takahashi, Kei, Naoe, Shiro, Suzuki, Kazuo, Ohno, Naohito
• Format: Article
• Language: English
• Subjects: Animals; Antibody Formation - immunology; Arteritis - chemically induced; Arteritis - immunology; Arteritis - pathology; Candida albicans; Candida albicans - chemistry; Candida albicans - immunology; Coronary Vessels - drug effects; Coronary Vessels - immunology; Coronary Vessels - pathology; Cytokines - immunology; DBA/2 mice; Disease Models, Animal; Enzyme-Linked Immunosorbent Assay; Induction of arteritis; Injections, Intraperitoneal; Male; Mice; Mice, Inbred C3H; Mice, Inbred C57BL; Mice, Inbred CBA; Mice, Inbred DBA; Polysaccharide; Polysaccharides - immunology; Polysaccharides - isolation & purification; Polysaccharides - toxicity; Species Specificity
• ISSN: 0892-3973; 1532-2513
• DOI: 10.1081/IPH-200042295

The intraperitoneal administration of CAWS (water-soluble extracellular polysaccharide fraction obtained from the culture supernatant of Candida albicans) to mice induces coronaritis similar to Kawasaki disease. We analyzed differences in the production of cytokines involved in the occurrence of coronary arteritis among mouse strains, C3H HeN, C57BL 6, DBA 2 and CBA J that were injected with CAWS at 4 mg mouse for 5 consecutive days in the first week and the fifth week of administration. The incidence of arteritis was 100% in C57BL 6, C3H HeN and DBA 2 mice, but only 10% in CBA J mice. The coronary arteritis observed in DBA 2 mice was the most serious, with several mice expiring during the observation period. The CAWS-sensitive strains revealed increased levels of IL-6 and IFN-γ during the course of a specific response to CAWS by spleen cells. In contrast, IL-10 levels were observed to increase markedly in CAWS-resistant CBA J mice, but not the CAWS-sensitive strains. However, TNF-α levels were more elevated only in DBA 2 mice. The difference in disease development and cytokine production strongly suggests that the genetic background of the immune response to CAWS contributes to the occurrence of coronary arteritis.