Internal transcribed spacer-2 restriction fragment length polymorphism (ITS-2-RFLP) tool to differentiate some exotic and indigenous trichogrammatid egg parasitoids in India

ITS-2-RFLP method was employed to distinguish 12 different trichogrammatids consisting of indigenous and exotic species such as Trichogrammatoidea armigera and Tr. bactrae, Trichogramma achaeae, T. chilonis, T. japonicum, T. embryophagum, T. pretiosum (Thelytokous Form—TF), T. brassicae, T. dendroli...

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Bibliographic Details
Published in:Biological control 2009-06, Vol.49 (3), p.207-213
Main Authors: Ashok Kumar, G., Jalali, Sushil K., Venkatesan, T., Stouthamer, Richard, Niranjana, P., Lalitha, Y.
Format: Article
Language:English
Subjects:
genetic variation
indigenous species
insect taxonomy
internal transcribed spacers
introduced species
ITS-2-RFLP
Molecular identification
molecular sequence data
molecular systematics
parasitoids
Restriction enzymes
restriction fragment length polymorphism
ribosomal DNA
sequence analysis
species differences
taxonomy
Trichogramma
Trichogrammatids
Trichogrammatoidea
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Summary:ITS-2-RFLP method was employed to distinguish 12 different trichogrammatids consisting of indigenous and exotic species such as Trichogrammatoidea armigera and Tr. bactrae, Trichogramma achaeae, T. chilonis, T. japonicum, T. embryophagum, T. pretiosum (Thelytokous Form—TF), T. brassicae, T. dendrolimi, T. evanescens and T. mwanzai. ITS-2 region was amplified; complete ITS-2 sequences of nine species were deposited in Genbank. The size of the amplified product ranged from 500 to 900 bp. Restriction enzyme digestion of ITS-2 region showed different banding profiles for these 12 species. Dichotomous keys using the size of the ITS-2 product and the restriction fragment length polymorphism for the enzymes (EcoRI, MseI, MvaI, and TaqI) allowed quick species identification of these trichogrammatids.
ISSN:1049-9644
1090-2112
DOI:10.1016/j.biocontrol.2009.02.010