DNA Methylation of Proximal PLAT Promoter in Chronic Rhinosinusitis With Nasal Polyps

Background Nasal polyps (NP) are characterized by pseudocysts derived from stromal tissue edema and cause persistent infections in patients with chronic rhinosinusitis (CRS). A low level of tissue-type plasminogen activator (gene name PLAT) is considered a cause of stromal tissue edema because of in...

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Published in:American journal of rhinology & allergy 2018-09, Vol.32 (5), p.374-379
Main Authors: Kidoguchi, Masanori, Noguchi, Emiko, Nakamura, Takako, Ninomiya, Takahiro, Morii, Wataru, Yoshida, Kanako, Morikawa, Taiyo, Kato, Yukinori, Imoto, Yoshimasa, Sakashita, Masafumi, Takabayashi, Tetsuji, Fujieda, Shigeharu
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Chronic Disease
DNA Methylation
Epigenesis, Genetic
Female
Gene Expression Regulation
Humans
Male
Middle Aged
Nasal Polyps - genetics
Promoter Regions, Genetic - genetics
Rhinitis - genetics
Sinusitis - genetics
Tissue Plasminogen Activator - genetics
Turbinates - physiology
Young Adult
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Summary:Background Nasal polyps (NP) are characterized by pseudocysts derived from stromal tissue edema and cause persistent infections in patients with chronic rhinosinusitis (CRS). A low level of tissue-type plasminogen activator (gene name PLAT) is considered a cause of stromal tissue edema because of insufficient plasmin activation in NP; however, the mechanism regulating PLAT gene expression levels is still unclear. The epigenetic mechanism regulating the PLAT gene expression has been studied in other tissues. Objective We aimed to investigate the methylation levels in the proximal PLAT promoter and their effects on gene expression in NP tissue. Methods We investigated the methylation levels at 3 CpG sites in the proximal PLAT promoter regions (−618, −121, and −105 with respect to the transcription initiation site) by bisulfite pyrosequencing and their effects on the gene expression by quantitative real-time polymerase chain reaction (qPCR) in 20 paired samples of NP and inferior turbinate tissue (IT) from patients with CRS. Results The DNA methylation levels at all CpG sites were higher (P 
ISSN:1945-8924
1945-8932
DOI:10.1177/1945892418782236