Cholera Toxin B Conjugated Quantum Dots for Live Cell Labeling

Cholera toxin subunit B (CTB)−quantum dot conjugates were developed for labeling mammalian cells. The conjugates were internalized by all tested cell lines into small vesicles dispersed throughout the cytoplasm, while commercially available polyarginine conjugates rapidly accumulated in large perinu...

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Bibliographic Details
Published in:Nano letters 2007-09, Vol.7 (9), p.2618-2626
Main Authors: Chakraborty, Subhasish K, Fitzpatrick, James A. J, Phillippi, Julie A, Andreko, Susan, Waggoner, Alan S, Bruchez, Marcel P, Ballou, Byron
Format: Article
Language:English
Subjects:
3T3 Cells
Animals
Applied sciences
Biological and medical sciences
Biotechnology
Cholera Toxin - chemistry
Cross-disciplinary physics: materials science
rheology
Crystallization - methods
Electronics
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Image Enhancement - methods
Materials science
Materials Testing
Methods. Procedures. Technologies
Mice
Microscopy, Fluorescence - methods
Molecular electronics, nanoelectronics
Nanocrystalline materials
Nanoscale materials and structures: fabrication and characterization
Nanotechnology - methods
Others
Particle Size
Physics
Quantum Dots
Semiconductor electronics. Microelectronics. Optoelectronics. Solid state devices
Staining and Labeling
Various methods and equipments
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Summary:Cholera toxin subunit B (CTB)−quantum dot conjugates were developed for labeling mammalian cells. The conjugates were internalized by all tested cell lines into small vesicles dispersed throughout the cytoplasm, while commercially available polyarginine conjugates rapidly accumulated in large perinuclear endosomes. Although a large proportion of CTB conjugates eventually also accumulated in perinuclear endosomes, this accumulation required several days, and even then many CTB conjugated quantum dots remained in small vesicles dispersed throughout the cytoplasm. Thus CTB conjugates are a practical alternative to polyarginine conjugates for the general labeling of mammalian cells.
ISSN:1530-6984
1530-6992
DOI:10.1021/nl0709930