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Alcohol Dehydrogenase Isoenzymes and Aldehyde Dehydrogenase Activity in the Serum of Patients with Non-alcoholic Fatty Liver Disease
Non-alcoholic liver disease (NAFLD) is one of the most common causes of chronic liver disease, and its prevalence and medical importance is increasing worldwide. Changes in enzyme activity in liver cells in various liver diseases are reflected by an increase in serum enzymatic activity. For example,...
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Published in: | Anticancer research 2018-07, Vol.38 (7), p.4005-4009 |
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creator | Jelski, Wojciech Wolszczak-Biedrzycka, Blanka Zasimowicz-Majewska, Elżbieta Orywal, Karolina Lapinski, Tadeusz Wojciech Szmitkowski, Maciej |
description | Non-alcoholic liver disease (NAFLD) is one of the most common causes of chronic liver disease, and its prevalence and medical importance is increasing worldwide. Changes in enzyme activity in liver cells in various liver diseases are reflected by an increase in serum enzymatic activity. For example, alcohol dehydrogenase activity (ADH) and aldehyde dehydrogenase (ALDH), that occur in the liver in large quantities, correlate with disease severity during cirrhosis. In the current study, the activity of ADH isoenzymes and ALDH in the serum of patients with NAFLD was investigated.
Serum samples were collected for routine biochemical studies from 55 patients with NAFLD patients and from 50 healthy individuals. Class I and II ADH and ALDH activity were measured by spectrofluorometric method. Photometric methods were used to measure ADH class III, IV and total ADH activity.
Total ADH activity was significantly higher in non-alcoholic fatty liver (NAFL) and non-alcoholic steatohepatitis (NASH) than in healthy individuals (44 and 48.5% activity, respectively). The median total activity of ADH was 1,164 mU/l in patients with NAFLD, 1,258 mU/l in NASH and 648 mU/l in the control group. The increase in ADH class I and II isoenzyme in serum of patients with NAFL and NASH was statistically significant. The activity of ADH I, ADH II, and total ADH significantly increased with increasing disease progression.
The activity of isozymes of class I and II alcohol dehydrogenase in patients with NAFLD is enhanced and appears to be due to the release of these isoenzymes from damaged hepatocytes. |
doi_str_mv | 10.21873/anticanres.12688 |
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Serum samples were collected for routine biochemical studies from 55 patients with NAFLD patients and from 50 healthy individuals. Class I and II ADH and ALDH activity were measured by spectrofluorometric method. Photometric methods were used to measure ADH class III, IV and total ADH activity.
Total ADH activity was significantly higher in non-alcoholic fatty liver (NAFL) and non-alcoholic steatohepatitis (NASH) than in healthy individuals (44 and 48.5% activity, respectively). The median total activity of ADH was 1,164 mU/l in patients with NAFLD, 1,258 mU/l in NASH and 648 mU/l in the control group. The increase in ADH class I and II isoenzyme in serum of patients with NAFL and NASH was statistically significant. The activity of ADH I, ADH II, and total ADH significantly increased with increasing disease progression.
The activity of isozymes of class I and II alcohol dehydrogenase in patients with NAFLD is enhanced and appears to be due to the release of these isoenzymes from damaged hepatocytes.</description><identifier>ISSN: 0250-7005</identifier><identifier>EISSN: 1791-7530</identifier><identifier>DOI: 10.21873/anticanres.12688</identifier><identifier>PMID: 29970524</identifier><language>eng</language><publisher>Greece: International Institute of Anticancer Research</publisher><subject>Alcohol ; Alcohol dehydrogenase ; Alcohols ; Aldehyde dehydrogenase ; Aldehydes ; Cirrhosis ; Dehydrogenase ; Dehydrogenases ; Enzymatic activity ; Enzyme activity ; Fatty liver ; Hepatocytes ; Isoenzymes ; Liver ; Liver cirrhosis ; Liver diseases ; Measurement methods ; Medical importance ; Patients ; Photometry ; Statistical analysis ; Statistical methods</subject><ispartof>Anticancer research, 2018-07, Vol.38 (7), p.4005-4009</ispartof><rights>Copyright© 2018, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.</rights><rights>Copyright International Institute of Anticancer Research Jul 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-771d5edc9ae16c38a558c56977fc4276bfa7126b8f65eaa1c2e16e95009f7a0b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29970524$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jelski, Wojciech</creatorcontrib><creatorcontrib>Wolszczak-Biedrzycka, Blanka</creatorcontrib><creatorcontrib>Zasimowicz-Majewska, Elżbieta</creatorcontrib><creatorcontrib>Orywal, Karolina</creatorcontrib><creatorcontrib>Lapinski, Tadeusz Wojciech</creatorcontrib><creatorcontrib>Szmitkowski, Maciej</creatorcontrib><title>Alcohol Dehydrogenase Isoenzymes and Aldehyde Dehydrogenase Activity in the Serum of Patients with Non-alcoholic Fatty Liver Disease</title><title>Anticancer research</title><addtitle>Anticancer Res</addtitle><description>Non-alcoholic liver disease (NAFLD) is one of the most common causes of chronic liver disease, and its prevalence and medical importance is increasing worldwide. Changes in enzyme activity in liver cells in various liver diseases are reflected by an increase in serum enzymatic activity. For example, alcohol dehydrogenase activity (ADH) and aldehyde dehydrogenase (ALDH), that occur in the liver in large quantities, correlate with disease severity during cirrhosis. In the current study, the activity of ADH isoenzymes and ALDH in the serum of patients with NAFLD was investigated.
Serum samples were collected for routine biochemical studies from 55 patients with NAFLD patients and from 50 healthy individuals. Class I and II ADH and ALDH activity were measured by spectrofluorometric method. Photometric methods were used to measure ADH class III, IV and total ADH activity.
Total ADH activity was significantly higher in non-alcoholic fatty liver (NAFL) and non-alcoholic steatohepatitis (NASH) than in healthy individuals (44 and 48.5% activity, respectively). The median total activity of ADH was 1,164 mU/l in patients with NAFLD, 1,258 mU/l in NASH and 648 mU/l in the control group. The increase in ADH class I and II isoenzyme in serum of patients with NAFL and NASH was statistically significant. The activity of ADH I, ADH II, and total ADH significantly increased with increasing disease progression.
The activity of isozymes of class I and II alcohol dehydrogenase in patients with NAFLD is enhanced and appears to be due to the release of these isoenzymes from damaged hepatocytes.</description><subject>Alcohol</subject><subject>Alcohol dehydrogenase</subject><subject>Alcohols</subject><subject>Aldehyde dehydrogenase</subject><subject>Aldehydes</subject><subject>Cirrhosis</subject><subject>Dehydrogenase</subject><subject>Dehydrogenases</subject><subject>Enzymatic activity</subject><subject>Enzyme activity</subject><subject>Fatty liver</subject><subject>Hepatocytes</subject><subject>Isoenzymes</subject><subject>Liver</subject><subject>Liver cirrhosis</subject><subject>Liver diseases</subject><subject>Measurement methods</subject><subject>Medical importance</subject><subject>Patients</subject><subject>Photometry</subject><subject>Statistical analysis</subject><subject>Statistical methods</subject><issn>0250-7005</issn><issn>1791-7530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNpdkclKBDEURYMo2g4f4EYCbtyUZugMtWycoVFBXRfp1Cs7UpVoklLatR9uaTuAqyzeufeFdxDapeSQUa34kfHZWeMjpEPKpNYraERVSQslOFlFI8IEKRQhYgNtpvRIiJSl5utog5WlIoKNR-h90towDy0-gfmijuEBvEmAL1MA_7boIGHjazxp688x_KMmNrsXlxfYeZzngG8h9h0ODb4x2YHPCb-6PMdXwRdmucZZfGbykJi6F4j4xCUYirbRWmPaBDvf7xa6Pzu9O74optfnl8eTaWG5YrlQitYCalsaoNJybYTQVshSqcaOmZKzxqjhDDPdSAHGUMsGDkpBSNkoQ2Z8Cx0se59ieO4h5apzyULbGg-hTxUjcsy4loIM6P4_9DH00Q-_qxjlmqgxF2Kg6JKyMaQUoameoutMXFSUVF-Kqj9F1ZeiIbP33dzPOqh_Ez9O-AfqwpDr</recordid><startdate>201807</startdate><enddate>201807</enddate><creator>Jelski, Wojciech</creator><creator>Wolszczak-Biedrzycka, Blanka</creator><creator>Zasimowicz-Majewska, Elżbieta</creator><creator>Orywal, Karolina</creator><creator>Lapinski, Tadeusz Wojciech</creator><creator>Szmitkowski, Maciej</creator><general>International Institute of Anticancer Research</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201807</creationdate><title>Alcohol Dehydrogenase Isoenzymes and Aldehyde Dehydrogenase Activity in the Serum of Patients with Non-alcoholic Fatty Liver Disease</title><author>Jelski, Wojciech ; Wolszczak-Biedrzycka, Blanka ; Zasimowicz-Majewska, Elżbieta ; Orywal, Karolina ; Lapinski, Tadeusz Wojciech ; Szmitkowski, Maciej</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-771d5edc9ae16c38a558c56977fc4276bfa7126b8f65eaa1c2e16e95009f7a0b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Alcohol</topic><topic>Alcohol dehydrogenase</topic><topic>Alcohols</topic><topic>Aldehyde dehydrogenase</topic><topic>Aldehydes</topic><topic>Cirrhosis</topic><topic>Dehydrogenase</topic><topic>Dehydrogenases</topic><topic>Enzymatic activity</topic><topic>Enzyme activity</topic><topic>Fatty liver</topic><topic>Hepatocytes</topic><topic>Isoenzymes</topic><topic>Liver</topic><topic>Liver cirrhosis</topic><topic>Liver diseases</topic><topic>Measurement methods</topic><topic>Medical importance</topic><topic>Patients</topic><topic>Photometry</topic><topic>Statistical analysis</topic><topic>Statistical methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jelski, Wojciech</creatorcontrib><creatorcontrib>Wolszczak-Biedrzycka, Blanka</creatorcontrib><creatorcontrib>Zasimowicz-Majewska, Elżbieta</creatorcontrib><creatorcontrib>Orywal, Karolina</creatorcontrib><creatorcontrib>Lapinski, Tadeusz Wojciech</creatorcontrib><creatorcontrib>Szmitkowski, Maciej</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Anticancer research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jelski, Wojciech</au><au>Wolszczak-Biedrzycka, Blanka</au><au>Zasimowicz-Majewska, Elżbieta</au><au>Orywal, Karolina</au><au>Lapinski, Tadeusz Wojciech</au><au>Szmitkowski, Maciej</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Alcohol Dehydrogenase Isoenzymes and Aldehyde Dehydrogenase Activity in the Serum of Patients with Non-alcoholic Fatty Liver Disease</atitle><jtitle>Anticancer research</jtitle><addtitle>Anticancer Res</addtitle><date>2018-07</date><risdate>2018</risdate><volume>38</volume><issue>7</issue><spage>4005</spage><epage>4009</epage><pages>4005-4009</pages><issn>0250-7005</issn><eissn>1791-7530</eissn><abstract>Non-alcoholic liver disease (NAFLD) is one of the most common causes of chronic liver disease, and its prevalence and medical importance is increasing worldwide. Changes in enzyme activity in liver cells in various liver diseases are reflected by an increase in serum enzymatic activity. For example, alcohol dehydrogenase activity (ADH) and aldehyde dehydrogenase (ALDH), that occur in the liver in large quantities, correlate with disease severity during cirrhosis. In the current study, the activity of ADH isoenzymes and ALDH in the serum of patients with NAFLD was investigated.
Serum samples were collected for routine biochemical studies from 55 patients with NAFLD patients and from 50 healthy individuals. Class I and II ADH and ALDH activity were measured by spectrofluorometric method. Photometric methods were used to measure ADH class III, IV and total ADH activity.
Total ADH activity was significantly higher in non-alcoholic fatty liver (NAFL) and non-alcoholic steatohepatitis (NASH) than in healthy individuals (44 and 48.5% activity, respectively). The median total activity of ADH was 1,164 mU/l in patients with NAFLD, 1,258 mU/l in NASH and 648 mU/l in the control group. The increase in ADH class I and II isoenzyme in serum of patients with NAFL and NASH was statistically significant. The activity of ADH I, ADH II, and total ADH significantly increased with increasing disease progression.
The activity of isozymes of class I and II alcohol dehydrogenase in patients with NAFLD is enhanced and appears to be due to the release of these isoenzymes from damaged hepatocytes.</abstract><cop>Greece</cop><pub>International Institute of Anticancer Research</pub><pmid>29970524</pmid><doi>10.21873/anticanres.12688</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Alcohol Alcohol dehydrogenase Alcohols Aldehyde dehydrogenase Aldehydes Cirrhosis Dehydrogenase Dehydrogenases Enzymatic activity Enzyme activity Fatty liver Hepatocytes Isoenzymes Liver Liver cirrhosis Liver diseases Measurement methods Medical importance Patients Photometry Statistical analysis Statistical methods |
title | Alcohol Dehydrogenase Isoenzymes and Aldehyde Dehydrogenase Activity in the Serum of Patients with Non-alcoholic Fatty Liver Disease |
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