Metal-Free All-Carbon Nanohybrid for Ultrasensitive Photoelectrochemical Immunosensing of alpha-Fetoprotein

C60 can accept up to six electrons reversibly and show exceptional light absorption over the entire UV–vis spectrum, making it a potential photoactive probe for photoelectrochemical (PEC) bioassay. However, few successful works have been reported to apply fullerenes in PEC biosensing, partially beca...

Full description

Saved in:
Bibliographic Details
Published in:ACS sensors 2018-07, Vol.3 (7), p.1385-1391
Main Authors: Zhou, Qing, Xue, Huaijia, Zhang, Yuye, Lv, Yanqin, Li, Hongguang, Liu, Songqin, Shen, Yanfei, Zhang, Yuanjian
Format: Article
Language:English
Subjects:
alpha-Fetoproteins - analysis
Antibodies, Immobilized - chemistry
Biosensing Techniques - methods
Electrochemical Techniques - methods
Fullerenes - chemistry
Graphite - chemistry
Humans
Immunoassay - methods
Limit of Detection
Nanostructures - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:C60 can accept up to six electrons reversibly and show exceptional light absorption over the entire UV–vis spectrum, making it a potential photoactive probe for photoelectrochemical (PEC) bioassay. However, few successful works have been reported to apply fullerenes in PEC biosensing, partially because of the low electronic conductivity and poor interfacial interactions with targeted biomolecules. Herein, we report the addressing of these two obstacles by coupling high conductive graphite flake (Gr), graphene oxide (GO) with sufficient oxygen-containing functional groups, and an alkylated C60 (AC60) into a metal-free all-carbon nanohybrid (AC60-Gr-GO) via harnessing delicate noncovalent interactions among them through a facile mechanical grinding. It was revealed that the as-obtained AC60-Gr-GO nanohybrid not only showed conspicuous enhancement of photocurrent up to 35 times but also offered rich anchors for bioconjugation. With detection of alpha-fetoprotein as an example, the AC60-Gr-GO based PEC immunosensor demonstrated a broad linear detection range (1 pg·mL–1 to 100 ng·mL–1) and a detection limit as low as 0.54 pg·mL–1, superior/competitive to PEC immunosensors for AFP in previous reports. By a proper reinforcement in conductivity and biointerface engineering, this work may provide a new way to use fullerenes as photoactive materials in more general PEC biosensing.
ISSN:2379-3694
2379-3694
DOI:10.1021/acssensors.8b00307