Functional genomics of calcium channels in human melanoma cells

Ca2+‐signaling of human melanoma is in the focus of intensive research since the identification of the role of WNT‐signaling in melanomagenesis. Genomic and functional studies pointed to the important role of various Ca2+ channels in melanoma, but these data were contradictory. In the present study...

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Published in:International journal of cancer 2007-07, Vol.121 (1), p.55-65
Main Authors: Deli, Tamás, Varga, Norbert, Ádám, Attila, Kenessey, István, Rásó, Erzsébet, Puskás, László G., Tóvári, József, Fodor, János, Fehér, Mónika, Szigeti, Gyula P., Csernoch, László, Tímár, József
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - pharmacology
apoptosis
Biological and medical sciences
Ca2+ transient
Calcium - metabolism
Cells, Cultured
Dermatology
Gene Expression Regulation, Neoplastic
Genome, Human - genetics
human melanoma
Humans
Medical sciences
Melanocytes - drug effects
Melanocytes - metabolism
Melanoma - genetics
Melanoma - metabolism
Nevus - genetics
P2X7 receptor
Receptors, Purinergic P2 - genetics
Receptors, Purinergic P2 - metabolism
Receptors, Purinergic P2X7
RNA, Small Interfering - genetics
Ryanodine Receptor Calcium Release Channel - genetics
Ryanodine Receptor Calcium Release Channel - metabolism
ryanodine receptor‐2
Tumors
Tumors of the skin and soft tissue. Premalignant lesions
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Summary:Ca2+‐signaling of human melanoma is in the focus of intensive research since the identification of the role of WNT‐signaling in melanomagenesis. Genomic and functional studies pointed to the important role of various Ca2+ channels in melanoma, but these data were contradictory. In the present study we clearly demonstrate, in a number of different ways including microarray analysis, DNA sequencing and immunocytochemistry, that various human melanoma cell lines and melanoma tissues overexpress ryanodine receptor type 2 (RyR2) and express P2X7 channel proteins as compared to melanocytes. These channels, although retain some of their usual characteristics and pharmacological properties, display unique features in melanoma cells, including a functional interaction between the two molecules. Unlike P2X7, RyR2 does not function as a calcium channel. On the other hand, the P2X7 receptor has an antiapoptotic function in melanoma cells, since ATP‐activation suppresses induced apoptosis, while knock down of the gene expression significantly enhances that. © 2007 Wiley‐Liss, Inc.
ISSN:0020-7136
1097-0215
DOI:10.1002/ijc.22621