Improved LC/MS Methods for the Analysis of Metal-Sensitive Analytes Using Medronic Acid as a Mobile Phase Additive

Phosphorylated compounds and organic acids with multiple carboxylate groups are commonly observed to have poor peak shapes and signal in LC/MS experiments. The poor peak shape is caused by the presence of trace metals, particularly iron, contributed from a variety of sources within the chromatograph...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2018-08, Vol.90 (15), p.9457-9464
Main Authors: Hsiao, Jordy J, Potter, Oscar G, Chu, Te-Wei, Yin, Hongfeng
Format: Article
Language:English
Subjects:
Additives
Analytical chemistry
Chemistry
Chromatography
Metabolites
Organic acids
Pesticides
Solvents
Trace metals
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Summary:Phosphorylated compounds and organic acids with multiple carboxylate groups are commonly observed to have poor peak shapes and signal in LC/MS experiments. The poor peak shape is caused by the presence of trace metals, particularly iron, contributed from a variety of sources within the chromatographic system. To ameliorate this problem, different solvent additives were investigated to reduce the amount of metal in the flow path to achieve better analytical performance for these metal-sensitive compounds. Here, we introduce the use of a solvent additive that can significantly improve the peak shapes and signal of metal-sensitive metabolites for LC/MS analysis. Moreover, the additive is shown to be amenable for other metal-sensitive applications, such as the analysis of phosphopeptides and polar phosphorylated pesticides, where the instruments could be used in either positive or negative analysis mode.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.8b02100