The effects of ethoxyquin and Angelica sinensis extracts on lipid oxidation in fish feeds and growth, digestive and absorptive capacities and antioxidant status in juvenile red carp (Cyprinus carpio var. xingguonensis): a comparative study

• Published in: Fish physiology and biochemistry 2019-02, Vol.45 (1), p.43-61
• Main Authors: Li, HuaTao, Tang, SiYi, Du, WenHao, Jiang, Jun, Peng, PeiYuan, Yuan, Ping, Liao, YiHong, Long, Jiao, Zhou, SiShun
• Format: Article
• Language: English
• Subjects: Absorptive capacity; Absorptivity; Acetates; Acetic acid; Alkaline phosphatase; Ammonia; Angelica sinensis; Angelica sinensis - chemistry; Animal Anatomy; Animal Biochemistry; Animal Feed - analysis; Animal Nutritional Physiological Phenomena; Animal Physiology; Animals; Anions; Antioxidants; Antioxidants - metabolism; Biomedical and Life Sciences; Carp; Carps - growth & development; Catalase; Comparative analysis; Comparative studies; Cyprinus carpio; Diet - veterinary; Digestion - drug effects; Emulsions; Ethanol; Ethoxyquin; Ethoxyquin - pharmacology; Ethyl acetate; Fish; Fish feeds; Freshwater & Marine Ecology; Freshwater fishes; Gene Expression Regulation - drug effects; Glutathione; Glutathione peroxidase; Glutathione reductase; Hepatopancreas; Histology; Hydroxyl radicals; Intestine; Intestines; Life Sciences; Linolenic acid; Lipase; Lipid peroxidation; Lipid Peroxidation - drug effects; Lipids; Malondialdehyde; Morphology; Oxidation; Peroxidase; Petroleum; Petroleum ether; Phosphatase; Plant Extracts - chemistry; Plant Extracts - pharmacology; Pyruvic acid; Random Allocation; Superoxide dismutase; Trypsin; Zoology; α-Amylase
• ISSN: 0920-1742; 1573-5168
• DOI: 10.1007/s10695-018-0533-x

Firstly, a linoleic and linolenic acid emulsion and fish feeds were incubated with graded levels of ethoxyquin (EQ) and petroleum ether extract, ethyl acetate extract (EAE), ethanol extract and aqueous extract of Angelica sinensis . The results showed that EQ and extracts of Angelica sinensis (EAs) inhibited lipid oxidation in material above. Of all of the examined EAs, EAE showed the strongest protective effects against the lipid oxidation. Moreover, EAE at high concentrations showed a stronger inhibitory effect on lipid oxidation than that of EQ. Next, 7 experimental diets that respectively supplemented 0.0, 0.2, 0.8 and 3.2 g kg −1 of EQ and EAE were fed to 280 juvenile red carp ( Cyprinus carpio var. xingguonensis) with seven treatment groups for 30 days. The results indicated that dietary EAE improved growth performance in carp. Moreover, dietary EAE increased the activities of trypsin, lipase, alpha-amylase, alkaline phosphatase, glutamate-oxaloacetate transaminase and glutamate-pyruvate transaminase (GPT) and decreased plasma ammonia content in carp. Meanwhile, dietary EAE reduced the levels of malondialdehyde and raised the activities of anti-superoxide anion, anti-hydroxyl radical, superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase and the content of reduced glutathione in the hepatopancreas and intestine of carp. However, with the exception of GPT, dietary EQ got the opposite results to dietary EAE in carp. These results revealed that dietary EAE improved the digestive, absorptive and antioxidant capacities in fish. However, dietary EQ inhibited the digestive, absorptive and antioxidant capacities in fish. So, EAE could be used as a natural antioxidant for replacing EQ in fish feeds.