Bacterial DNA activates immunity in Arabidopsis thaliana

To initiate defense responses against invasion of pathogenic organisms, animals and plants must recognize microbe-associated molecular patterns (MAMPs). In this study, the elicitor activity of bacterial DNA on the model plant Arabidopsis thaliana was examined. EcoRI-digested plasmid DNA induced defe...

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Bibliographic Details
Published in:Journal of general plant pathology : JGPP 2009-06, Vol.75 (3), p.227-234
Main Authors: Yakushiji, S.(Okayama Univ. (Japan)), Ishiga, Y, Inagaki, Y, Toyoda, K, Shiraishi, T, Ichinose, Y
Format: Article
Language:English
Subjects:
ADN
Agriculture
ARABIDOPSIS THALIANA
BACTERIA
Bacterial and Phytoplasma Diseases
BIOLOGIA MOLECULAR
BIOLOGIE MOLECULAIRE
Biomedical and Life Sciences
CONTROL DE ENFERMEDADES
CONTROLE DE MALADIES
DEFENCE MECHANISMS
Deoxyribonucleic acid
DISEASE CONTROL
DNA
E coli
ENDOCITOSIS
ENDOCYTOSE
ENDOCYTOSIS
Escherichia coli
Flowers & plants
Life Sciences
MECANISME DE DEFENSE
MECANISMOS DE DEFENSA
Microbiology
MOLECULAR BIOLOGY
Pathogens
Plant Pathology
Plant resistance
PLASMIDE
PLASMIDIOS
PLASMIDS
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Summary:To initiate defense responses against invasion of pathogenic organisms, animals and plants must recognize microbe-associated molecular patterns (MAMPs). In this study, the elicitor activity of bacterial DNA on the model plant Arabidopsis thaliana was examined. EcoRI-digested plasmid DNA induced defense responses such as generation of reactive oxygen species and deposition of callose, whereas SmaI- and HapII-digested plasmid DNA and EcoRI-digested herring DNA did not remarkably induce these responses. Further, methylation of the CpG sequence of plasmid DNA and Escherichia coli DNA reduced the level of the defense responses. The endocytosis inhibitors wortmannin and amantadine significantly inhibited DNA-induced defense responses. These results suggest that nonmethylated CpG DNA, as a MAMP, induced defense responses in Arabidopsis and that non-methylated DNA seems to be translocated into the cytoplasm by endocytosis.
ISSN:1345-2630
1610-739X
DOI:10.1007/s10327-009-0162-4