Loading…

Bacterial DNA activates immunity in Arabidopsis thaliana

To initiate defense responses against invasion of pathogenic organisms, animals and plants must recognize microbe-associated molecular patterns (MAMPs). In this study, the elicitor activity of bacterial DNA on the model plant Arabidopsis thaliana was examined. EcoRI-digested plasmid DNA induced defe...

Full description

Saved in:

Bibliographic Details
Published in:	Journal of general plant pathology : JGPP 2009-06, Vol.75 (3), p.227-234
Main Authors:	Yakushiji, S.(Okayama Univ. (Japan)), Ishiga, Y, Inagaki, Y, Toyoda, K, Shiraishi, T, Ichinose, Y
Format:	Article
Language:	English
Subjects:	ADN Agriculture ARABIDOPSIS THALIANA BACTERIA Bacterial and Phytoplasma Diseases BIOLOGIA MOLECULAR BIOLOGIE MOLECULAIRE Biomedical and Life Sciences CONTROL DE ENFERMEDADES CONTROLE DE MALADIES DEFENCE MECHANISMS Deoxyribonucleic acid DISEASE CONTROL DNA E coli ENDOCITOSIS ENDOCYTOSE ENDOCYTOSIS Escherichia coli Flowers & plants Life Sciences MECANISME DE DEFENSE MECANISMOS DE DEFENSA Microbiology MOLECULAR BIOLOGY Pathogens Plant Pathology Plant resistance PLASMIDE PLASMIDIOS PLASMIDS
Citations:	Items that this one cites Items that cite this one
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

cited_by	cdi_FETCH-LOGICAL-c397t-90556f14cde125fb8a80844cda01124a5f133e7399596ee6b5352044f0c40d6d3
cites	cdi_FETCH-LOGICAL-c397t-90556f14cde125fb8a80844cda01124a5f133e7399596ee6b5352044f0c40d6d3
container_end_page	234
container_issue	3
container_start_page	227
container_title	Journal of general plant pathology : JGPP
container_volume	75
creator	Yakushiji, S.(Okayama Univ. (Japan)) Ishiga, Y Inagaki, Y Toyoda, K Shiraishi, T Ichinose, Y
description	To initiate defense responses against invasion of pathogenic organisms, animals and plants must recognize microbe-associated molecular patterns (MAMPs). In this study, the elicitor activity of bacterial DNA on the model plant Arabidopsis thaliana was examined. EcoRI-digested plasmid DNA induced defense responses such as generation of reactive oxygen species and deposition of callose, whereas SmaI- and HapII-digested plasmid DNA and EcoRI-digested herring DNA did not remarkably induce these responses. Further, methylation of the CpG sequence of plasmid DNA and Escherichia coli DNA reduced the level of the defense responses. The endocytosis inhibitors wortmannin and amantadine significantly inhibited DNA-induced defense responses. These results suggest that nonmethylated CpG DNA, as a MAMP, induced defense responses in Arabidopsis and that non-methylated DNA seems to be translocated into the cytoplasm by endocytosis.
doi_str_mv	10.1007/s10327-009-0162-4
format	article
fullrecord	<record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_20667341</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>20667341</sourcerecordid><originalsourceid>FETCH-LOGICAL-c397t-90556f14cde125fb8a80844cda01124a5f133e7399596ee6b5352044f0c40d6d3</originalsourceid><addsrcrecordid>eNp9kEtLxDAUhYMoOI7-ABdCceEuem9ebZfjWxnUhYK7kGnTMUMfY9IK8-_NUEFw4ereA985HA4hxwjnCJBeBATOUgqQU0DFqNghE1QINOX5-278uZCUKQ775CCEFQBDnsoJyS5N0VvvTJ1cP82SKNyX6W1IXNMMres3iWuTmTcLV3br4ELSf5jamdYckr3K1MEe_dwpebu9eb26p_Pnu4er2ZwWPE97moOUqkJRlBaZrBaZySATURpAZMLICjm3sWQuc2WtWkguGQhRQSGgVCWfkrMxd-27z8GGXjcuFLauTWu7IWgGSqVcYARP_4CrbvBt7KYZZkqKlGURwhEqfBeCt5Vee9cYv9EIejukHofUcUi9HVKL6GGjJ0S2XVr_G_yf6WQ0VabTZuld0I8vLAIAIgXGvwGstXwO</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>218654728</pqid></control><display><type>article</type><title>Bacterial DNA activates immunity in Arabidopsis thaliana</title><source>Springer Link</source><creator>Yakushiji, S.(Okayama Univ. (Japan)) ; Ishiga, Y ; Inagaki, Y ; Toyoda, K ; Shiraishi, T ; Ichinose, Y</creator><creatorcontrib>Yakushiji, S.(Okayama Univ. (Japan)) ; Ishiga, Y ; Inagaki, Y ; Toyoda, K ; Shiraishi, T ; Ichinose, Y</creatorcontrib><description>To initiate defense responses against invasion of pathogenic organisms, animals and plants must recognize microbe-associated molecular patterns (MAMPs). In this study, the elicitor activity of bacterial DNA on the model plant Arabidopsis thaliana was examined. EcoRI-digested plasmid DNA induced defense responses such as generation of reactive oxygen species and deposition of callose, whereas SmaI- and HapII-digested plasmid DNA and EcoRI-digested herring DNA did not remarkably induce these responses. Further, methylation of the CpG sequence of plasmid DNA and Escherichia coli DNA reduced the level of the defense responses. The endocytosis inhibitors wortmannin and amantadine significantly inhibited DNA-induced defense responses. These results suggest that nonmethylated CpG DNA, as a MAMP, induced defense responses in Arabidopsis and that non-methylated DNA seems to be translocated into the cytoplasm by endocytosis.</description><identifier>ISSN: 1345-2630</identifier><identifier>EISSN: 1610-739X</identifier><identifier>DOI: 10.1007/s10327-009-0162-4</identifier><identifier>CODEN: JGPPBQ</identifier><language>eng</language><publisher>Japan: Springer Japan</publisher><subject>ADN ; Agriculture ; ARABIDOPSIS THALIANA ; BACTERIA ; Bacterial and Phytoplasma Diseases ; BIOLOGIA MOLECULAR ; BIOLOGIE MOLECULAIRE ; Biomedical and Life Sciences ; CONTROL DE ENFERMEDADES ; CONTROLE DE MALADIES ; DEFENCE MECHANISMS ; Deoxyribonucleic acid ; DISEASE CONTROL ; DNA ; E coli ; ENDOCITOSIS ; ENDOCYTOSE ; ENDOCYTOSIS ; Escherichia coli ; Flowers & plants ; Life Sciences ; MECANISME DE DEFENSE ; MECANISMOS DE DEFENSA ; Microbiology ; MOLECULAR BIOLOGY ; Pathogens ; Plant Pathology ; Plant resistance ; PLASMIDE ; PLASMIDIOS ; PLASMIDS</subject><ispartof>Journal of general plant pathology : JGPP, 2009-06, Vol.75 (3), p.227-234</ispartof><rights>The Phytopathological Society of Japan and Springer 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c397t-90556f14cde125fb8a80844cda01124a5f133e7399596ee6b5352044f0c40d6d3</citedby><cites>FETCH-LOGICAL-c397t-90556f14cde125fb8a80844cda01124a5f133e7399596ee6b5352044f0c40d6d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Yakushiji, S.(Okayama Univ. (Japan))</creatorcontrib><creatorcontrib>Ishiga, Y</creatorcontrib><creatorcontrib>Inagaki, Y</creatorcontrib><creatorcontrib>Toyoda, K</creatorcontrib><creatorcontrib>Shiraishi, T</creatorcontrib><creatorcontrib>Ichinose, Y</creatorcontrib><title>Bacterial DNA activates immunity in Arabidopsis thaliana</title><title>Journal of general plant pathology : JGPP</title><addtitle>J Gen Plant Pathol</addtitle><description>To initiate defense responses against invasion of pathogenic organisms, animals and plants must recognize microbe-associated molecular patterns (MAMPs). In this study, the elicitor activity of bacterial DNA on the model plant Arabidopsis thaliana was examined. EcoRI-digested plasmid DNA induced defense responses such as generation of reactive oxygen species and deposition of callose, whereas SmaI- and HapII-digested plasmid DNA and EcoRI-digested herring DNA did not remarkably induce these responses. Further, methylation of the CpG sequence of plasmid DNA and Escherichia coli DNA reduced the level of the defense responses. The endocytosis inhibitors wortmannin and amantadine significantly inhibited DNA-induced defense responses. These results suggest that nonmethylated CpG DNA, as a MAMP, induced defense responses in Arabidopsis and that non-methylated DNA seems to be translocated into the cytoplasm by endocytosis.</description><subject>ADN</subject><subject>Agriculture</subject><subject>ARABIDOPSIS THALIANA</subject><subject>BACTERIA</subject><subject>Bacterial and Phytoplasma Diseases</subject><subject>BIOLOGIA MOLECULAR</subject><subject>BIOLOGIE MOLECULAIRE</subject><subject>Biomedical and Life Sciences</subject><subject>CONTROL DE ENFERMEDADES</subject><subject>CONTROLE DE MALADIES</subject><subject>DEFENCE MECHANISMS</subject><subject>Deoxyribonucleic acid</subject><subject>DISEASE CONTROL</subject><subject>DNA</subject><subject>E coli</subject><subject>ENDOCITOSIS</subject><subject>ENDOCYTOSE</subject><subject>ENDOCYTOSIS</subject><subject>Escherichia coli</subject><subject>Flowers & plants</subject><subject>Life Sciences</subject><subject>MECANISME DE DEFENSE</subject><subject>MECANISMOS DE DEFENSA</subject><subject>Microbiology</subject><subject>MOLECULAR BIOLOGY</subject><subject>Pathogens</subject><subject>Plant Pathology</subject><subject>Plant resistance</subject><subject>PLASMIDE</subject><subject>PLASMIDIOS</subject><subject>PLASMIDS</subject><issn>1345-2630</issn><issn>1610-739X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNp9kEtLxDAUhYMoOI7-ABdCceEuem9ebZfjWxnUhYK7kGnTMUMfY9IK8-_NUEFw4ereA985HA4hxwjnCJBeBATOUgqQU0DFqNghE1QINOX5-278uZCUKQ775CCEFQBDnsoJyS5N0VvvTJ1cP82SKNyX6W1IXNMMres3iWuTmTcLV3br4ELSf5jamdYckr3K1MEe_dwpebu9eb26p_Pnu4er2ZwWPE97moOUqkJRlBaZrBaZySATURpAZMLICjm3sWQuc2WtWkguGQhRQSGgVCWfkrMxd-27z8GGXjcuFLauTWu7IWgGSqVcYARP_4CrbvBt7KYZZkqKlGURwhEqfBeCt5Vee9cYv9EIejukHofUcUi9HVKL6GGjJ0S2XVr_G_yf6WQ0VabTZuld0I8vLAIAIgXGvwGstXwO</recordid><startdate>20090601</startdate><enddate>20090601</enddate><creator>Yakushiji, S.(Okayama Univ. (Japan))</creator><creator>Ishiga, Y</creator><creator>Inagaki, Y</creator><creator>Toyoda, K</creator><creator>Shiraishi, T</creator><creator>Ichinose, Y</creator><general>Springer Japan</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7TM</scope></search><sort><creationdate>20090601</creationdate><title>Bacterial DNA activates immunity in Arabidopsis thaliana</title><author>Yakushiji, S.(Okayama Univ. (Japan)) ; Ishiga, Y ; Inagaki, Y ; Toyoda, K ; Shiraishi, T ; Ichinose, Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c397t-90556f14cde125fb8a80844cda01124a5f133e7399596ee6b5352044f0c40d6d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>ADN</topic><topic>Agriculture</topic><topic>ARABIDOPSIS THALIANA</topic><topic>BACTERIA</topic><topic>Bacterial and Phytoplasma Diseases</topic><topic>BIOLOGIA MOLECULAR</topic><topic>BIOLOGIE MOLECULAIRE</topic><topic>Biomedical and Life Sciences</topic><topic>CONTROL DE ENFERMEDADES</topic><topic>CONTROLE DE MALADIES</topic><topic>DEFENCE MECHANISMS</topic><topic>Deoxyribonucleic acid</topic><topic>DISEASE CONTROL</topic><topic>DNA</topic><topic>E coli</topic><topic>ENDOCITOSIS</topic><topic>ENDOCYTOSE</topic><topic>ENDOCYTOSIS</topic><topic>Escherichia coli</topic><topic>Flowers & plants</topic><topic>Life Sciences</topic><topic>MECANISME DE DEFENSE</topic><topic>MECANISMOS DE DEFENSA</topic><topic>Microbiology</topic><topic>MOLECULAR BIOLOGY</topic><topic>Pathogens</topic><topic>Plant Pathology</topic><topic>Plant resistance</topic><topic>PLASMIDE</topic><topic>PLASMIDIOS</topic><topic>PLASMIDS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yakushiji, S.(Okayama Univ. (Japan))</creatorcontrib><creatorcontrib>Ishiga, Y</creatorcontrib><creatorcontrib>Inagaki, Y</creatorcontrib><creatorcontrib>Toyoda, K</creatorcontrib><creatorcontrib>Shiraishi, T</creatorcontrib><creatorcontrib>Ichinose, Y</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>ProQuest Research Library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Journal of general plant pathology : JGPP</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yakushiji, S.(Okayama Univ. (Japan))</au><au>Ishiga, Y</au><au>Inagaki, Y</au><au>Toyoda, K</au><au>Shiraishi, T</au><au>Ichinose, Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bacterial DNA activates immunity in Arabidopsis thaliana</atitle><jtitle>Journal of general plant pathology : JGPP</jtitle><stitle>J Gen Plant Pathol</stitle><date>2009-06-01</date><risdate>2009</risdate><volume>75</volume><issue>3</issue><spage>227</spage><epage>234</epage><pages>227-234</pages><issn>1345-2630</issn><eissn>1610-739X</eissn><coden>JGPPBQ</coden><abstract>To initiate defense responses against invasion of pathogenic organisms, animals and plants must recognize microbe-associated molecular patterns (MAMPs). In this study, the elicitor activity of bacterial DNA on the model plant Arabidopsis thaliana was examined. EcoRI-digested plasmid DNA induced defense responses such as generation of reactive oxygen species and deposition of callose, whereas SmaI- and HapII-digested plasmid DNA and EcoRI-digested herring DNA did not remarkably induce these responses. Further, methylation of the CpG sequence of plasmid DNA and Escherichia coli DNA reduced the level of the defense responses. The endocytosis inhibitors wortmannin and amantadine significantly inhibited DNA-induced defense responses. These results suggest that nonmethylated CpG DNA, as a MAMP, induced defense responses in Arabidopsis and that non-methylated DNA seems to be translocated into the cytoplasm by endocytosis.</abstract><cop>Japan</cop><pub>Springer Japan</pub><doi>10.1007/s10327-009-0162-4</doi><tpages>8</tpages></addata></record>
fulltext	fulltext
identifier	ISSN: 1345-2630
ispartof	Journal of general plant pathology : JGPP, 2009-06, Vol.75 (3), p.227-234
issn	1345-2630 1610-739X
language	eng
recordid	cdi_proquest_miscellaneous_20667341
source	Springer Link
subjects	ADN Agriculture ARABIDOPSIS THALIANA BACTERIA Bacterial and Phytoplasma Diseases BIOLOGIA MOLECULAR BIOLOGIE MOLECULAIRE Biomedical and Life Sciences CONTROL DE ENFERMEDADES CONTROLE DE MALADIES DEFENCE MECHANISMS Deoxyribonucleic acid DISEASE CONTROL DNA E coli ENDOCITOSIS ENDOCYTOSE ENDOCYTOSIS Escherichia coli Flowers & plants Life Sciences MECANISME DE DEFENSE MECANISMOS DE DEFENSA Microbiology MOLECULAR BIOLOGY Pathogens Plant Pathology Plant resistance PLASMIDE PLASMIDIOS PLASMIDS
title	Bacterial DNA activates immunity in Arabidopsis thaliana
url	http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T01%3A52%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Bacterial%20DNA%20activates%20immunity%20in%20Arabidopsis%20thaliana&rft.jtitle=Journal%20of%20general%20plant%20pathology%20:%20JGPP&rft.au=Yakushiji,%20S.(Okayama%20Univ.%20(Japan))&rft.date=2009-06-01&rft.volume=75&rft.issue=3&rft.spage=227&rft.epage=234&rft.pages=227-234&rft.issn=1345-2630&rft.eissn=1610-739X&rft.coden=JGPPBQ&rft_id=info:doi/10.1007/s10327-009-0162-4&rft_dat=%3Cproquest_cross%3E20667341%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c397t-90556f14cde125fb8a80844cda01124a5f133e7399596ee6b5352044f0c40d6d3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=218654728&rft_id=info:pmid/&rfr_iscdi=true