Production and properties of bacterial cellulose by the strain Komagataeibacter xylinus B-12068

A strain of acetic acid bacteria, Komagataeibacter xylinus B-12068, was studied as a source for bacterial cellulose (BC) production. The effects of cultivation conditions (carbon sources, temperature, and pH) on BC production and properties were studied in surface and submerged cultures. Glucose was...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2018-09, Vol.102 (17), p.7417-7428
Main Authors: Volova, Tatiana G., Prudnikova, Svetlana V., Sukovatyi, Aleksey G., Shishatskaya, Ekaterina I.
Format: Article
Language:English
Subjects:
Acetic acid
Acetic acid bacteria
Analysis
Animals
Bacteria
Biocompatibility
Biomedical and Life Sciences
Biotechnological Products and Process Engineering
Biotechnology
Carbon sources
Cellulose
Cellulose - biosynthesis
Cultivation
Cytotoxicity
Emission analysis
Ethanol
Ethanol - metabolism
Gluconacetobacter xylinus - metabolism
Glucose
Glucose - metabolism
Glycerol
Hydrogen-Ion Concentration
Industrial Microbiology - methods
Life Sciences
Mechanical properties
Methods
Microbial Genetics and Genomics
Microbiological synthesis
Microbiology
Physical properties
Physiological aspects
Properties
Proteobacteria
Spectrometry
Substrates
Sugar
Temperature
Toxicity
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Summary:A strain of acetic acid bacteria, Komagataeibacter xylinus B-12068, was studied as a source for bacterial cellulose (BC) production. The effects of cultivation conditions (carbon sources, temperature, and pH) on BC production and properties were studied in surface and submerged cultures. Glucose was found to be the best substrate for BC production among the sugars tested; ethanol concentration of 3% ( w / v ) enhanced the productivity of BC. Optimization of medium and cultivation conditions ensures a high production of BC on glucose and glycerol, up to 2.4 and 3.3 g/L/day, respectively. C/N elemental analysis, emission spectrometry, SEM, DTA, and X-ray were used to investigate the structure and physical and mechanical properties of the BC produced under different conditions. MTT assay and SEM showed that native cellulose membrane did not cause cytotoxicity upon direct contact with NIH 3T3 mouse fibroblast cells and was highly biocompatible.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-018-9198-8