Modulation of carcinogen bioavailability by immunisation with benzo[ a ]pyrene-conjugate vaccines

Abstract Benzo[ a ]pyrene (B[ a ]P) conjugate vaccines based on ovalbumin, tetanus toxoid and diphtheria toxoid (DT) as carrier proteins were developed to investigate the effect of specific antibodies on the bioavailability of this ubiquitous carcinogen and its metabolites. After metabolic activatio...

Full description

Saved in:
Bibliographic Details
Published in:Vaccine 2009-06, Vol.27 (31), p.4142-4151
Main Authors: Grova, Nathalie, Prodhomme, Emmanuel J.F, Schellenberger, Mario T, Farinelle, Sophie, Muller, Claude P
Format: Article
Language:English
Subjects:
Allergy and Immunology
Animals
Applied microbiology
Benzo(a)pyrene - antagonists & inhibitors
Benzo[ a]pyrene
Binding sites
Bioavailability
Biological and medical sciences
Biological Availability
Carcinogenesis
Carcinogens
Carcinogens - antagonists & inhibitors
Competition
Cross Reactions
Deoxyribonucleic acid
DNA
DNA repair
Female
Fundamental and applied biological sciences. Psychology
Immune response
Immunization
Medical sciences
Metabolism
Metabolites
Mice
Mice, Inbred BALB C
Microbiology
Molecular weight
Pharmacokinetics
Proteins
Specific antibodies
Tumors
Vaccines
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
Vaccines, Conjugate - immunology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Benzo[ a ]pyrene (B[ a ]P) conjugate vaccines based on ovalbumin, tetanus toxoid and diphtheria toxoid (DT) as carrier proteins were developed to investigate the effect of specific antibodies on the bioavailability of this ubiquitous carcinogen and its metabolites. After metabolic activation of this prototype carcinogen, B[ a ]P forms DNA adducts which initiate chemical carcinogenesis. B[ a ]P-DT conjugate induced the most robust immune response. The antibodies reacted not only with B[ a ]P but also with the proximate carcinogen 7,8-diol-B[ a ]P. Antibodies modulated the bioavailability of B[ a ]P and its metabolic activation in a dose-dependent manner by sequestration in the blood. Our results showed that this immune prophylactic strategy influences the pharmacokinetic of B[ a ]P and further studies to investigate their effects on chemical carcinogenesis are warranted.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2009.04.052