Ovine vital neutrophil extracellular traps bind and impair Haemonchus contortus L3 in a breed‐dependent manner

Summary This study aimed to characterize neutrophil response to Haemonchus contortus (Hc) in vitro using cells from parasite‐resistant St. Croix (STC) and parasite‐susceptible Suffolk (SUF) sheep. Neutrophils from Hc‐primed and naive STC and SUF sheep were incubated with Hc larval antigen (HcLA), Hc...

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Bibliographic Details
Published in:Parasite immunology 2018-09, Vol.40 (9), p.e12572-n/a
Main Authors: Garza, Javier J., Greiner, Scott P., Bowdridge, Scott A.
Format: Article
Language:English
Subjects:
Antigens
apoptosis
Deoxyribonucleic acid
DNA
extracellular traps
Haemonchus
Haemonchus contortus
helminths
immune system
larva
Leukocytes (neutrophilic)
Neutrophils
Ovis aries
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Summary:Summary This study aimed to characterize neutrophil response to Haemonchus contortus (Hc) in vitro using cells from parasite‐resistant St. Croix (STC) and parasite‐susceptible Suffolk (SUF) sheep. Neutrophils from Hc‐primed and naive STC and SUF sheep were incubated with Hc larval antigen (HcLA), Hc worm antigen (HcWA) or complete media (CM). After HcLA exposure, neutrophils from STC and SUF formed extracellular traps composed of DNA. Stimulation with HcLA induced a 35‐fold increase in extracellular DNA compared to CM controls. However, extracellular DNA was not found when neutrophils were cultured with HcWA. The formation of neutrophil extracellular traps (NET) in response to HcLA yields a low percentage of necrotic cells indicating a form of vital NETosis. Neutrophils from primed and naïve STC bound Hc L3 greater (93% and 68%) than SUF (78% and 45%; P 
ISSN:0141-9838
1365-3024
DOI:10.1111/pim.12572