In vitro evaluation of biomarkers of nephrotoxicity through gene expression using gentamicin

Acute renal failure is one of the most frequent effects observed after taking medicine. Such situations have been tardily discovered, given that existing methods for assessing toxicity are not predictive. In this light, the present work evaluated the effects of gentamicin, a form of nephrotoxic drug...

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Bibliographic Details
Published in:Journal of biochemical and molecular toxicology 2018-09, Vol.32 (9), p.e22189-n/a
Main Authors: Campos, Maria A. A., Almeida, Leonardo A., Grossi, Marina F., Tagliati, Carlos A.
Format: Article
Language:English
Subjects:
Apoptosis
Biomarkers
Cytotoxicity
Flow cytometry
Gene expression
Genes
Gentamicin
Necrosis
nephrotoxicity
p53 Protein
Renal failure
Toxicity
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Summary:Acute renal failure is one of the most frequent effects observed after taking medicine. Such situations have been tardily discovered, given that existing methods for assessing toxicity are not predictive. In this light, the present work evaluated the effects of gentamicin, a form of nephrotoxic drug, on HK‐2 and HEK‐293 cells. By using MTT (3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide) and flow cytometry, both cells demonstrated that cytotoxicity occurs in a dose‐dependent manner through the processes of apoptosis and cell necrosis. Gene expression analysis showed a relative increase of expression for genes related to cell processes and classic biomarkers, such as TP53, CASP3, CASP8, CASP9, ICAM‐1, EXOC3, KIM‐1, and CST3. A decrease in expression for genes BCL2L1 and EGF was observed. This study, therefore, indicates that, when the methods are used together, gene expression analysis is able to evaluate the nephrotoxic potential of a substance.
ISSN:1095-6670
1099-0461
DOI:10.1002/jbt.22189