Enzymatic detection of As(III) in aqueous solution using alginate immobilized pumpkin urease: Optimization of process variables by response surface methodology

Urease immobilized on alginate was utilized to detect and quantify As 3+ in aqueous solution. Urease from the seeds of pumpkin (vegetable waste) was purified to apparent homogeneity by heat treatment and gel filtration (Sephadex G-200). Further enzyme was entrapped in 3.5% alginate beads. Urea hydro...

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Bibliographic Details
Published in:Bioresource technology 2009-10, Vol.100 (19), p.4462-4467
Main Authors: Talat, Mahe, Prakash, Om, Hasan, S.H.
Format: Article
Language:English
Subjects:
agricultural wastes
alginates
Alginates - chemistry
Analysis of Variance
aqueous solutions
arsenic
Arsenic - analysis
As 3
Biological and medical sciences
chemical analysis
Cucurbita - enzymology
Environmental Restoration and Remediation - methods
enzymatic hydrolysis
enzyme activity
Enzymes, Immobilized - metabolism
Fundamental and applied biological sciences. Psychology
Glucuronic Acid - chemistry
groundwater
Hexuronic Acids - chemistry
Immobilized
immobilized enzymes
Inhibition
Ions
plant residues
pollutants
pumpkins
quantitative analysis
Regression Analysis
Response surface methodology
seeds
Solubility
Solutions
Substrate Specificity
Surface Properties
Time Factors
toxic substances
urea
Urease
Urease - metabolism
Water - chemistry
water pollution
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Summary:Urease immobilized on alginate was utilized to detect and quantify As 3+ in aqueous solution. Urease from the seeds of pumpkin (vegetable waste) was purified to apparent homogeneity by heat treatment and gel filtration (Sephadex G-200). Further enzyme was entrapped in 3.5% alginate beads. Urea hydrolysis by enzyme revealed a clear dependence on the concentration and interaction time of As 3+. The process variables effecting the quantitation of As 3+ was investigated using central composite design with Minitab ® 15 software. The predicted results were found in good agreement ( R 2 = 96.71%) with experimental results indicating the applicability of proposed model. The multiple regression analysis and ANOVA showed that enzyme activity decreased with increase of As 3+ concentration and interaction time. 3D plot and contour plot between As 3+ concentration and interaction time was helpful to predict residual activity of enzyme for a particular As 3+ at a particular time.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2009.04.009