Secretion of a potent antibiotic by salt-tolerant and alkaliphilic actinomycete Streptomyces sannanensis strain RJT-1

An alkaliphilic actinomycete, Streptomyces sannanensis strain RJT-1 was isolated from the alkaline soil of Saurashtra University Campus, Rajkot. The isolate exhibited optimal growth at 5% (w/v) NaCl and pH 9. It was Gram-positive, having filamentous, long thread-like structure and started sporulatio...

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Bibliographic Details
Published in:Current science (Bangalore) 2006-11, Vol.91 (10), p.1393-1397
Main Authors: Vasavada, S. H., Thumar, J. T., Singh, S. P.
Format: Article
Language:English
Subjects:
Actinomyces
Actinomycetes
Alkaline soils
Antibiotics
Antimicrobials
Enzymes
Nitrogen
RESEARCH COMMUNICATIONS
Starches
Streptomyces
Table salt
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Summary:An alkaliphilic actinomycete, Streptomyces sannanensis strain RJT-1 was isolated from the alkaline soil of Saurashtra University Campus, Rajkot. The isolate exhibited optimal growth at 5% (w/v) NaCl and pH 9. It was Gram-positive, having filamentous, long thread-like structure and started sporulation after 3 days of incubation. It was capable of producing antibiotic against Gram-positive bacteria, while Gram-negative organisms were not affected. Starch agar was the preferred medium for antibiotic production. Optimum salt and pH for antibiotic production were 3% (w/v) NaCl and 9 respectively. Studies on the nutritional factors showed that the highest antibacterial activity was obtained when glucose and lactose at 1% (w/v) were used as carbon sources. S. sannanensis strain RJT-1 displayed higher antibiotic production with inorganic nitrogen sources such as urea, (NH₄)₂SO₄ and NH₄NO₃ compared to organic nitrogen supplements. So far only few alkaliphilic actinomycetes have been explored for their antimicrobial potential.
ISSN:0011-3891