Tolerance, accumulation and distribution of zinc and cadmium in hyperaccumulator Potentilla griffithii

• Published in: Environmental and experimental botany 2009-05, Vol.66 (2), p.317-325
• Main Authors: Hu, Peng-Jie, Qiu, Rong-Liang, Senthilkumar, Palaninaicker, Jiang, Dan, Chen, Zhong-Wei, Tang, Ye-Tao, Liu, Feng-Jie
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Biomass; Cadmium; Distribution; Fundamental and applied biological sciences. Psychology; Hyperaccumulation; Microscopy; Potentilla; Potentilla griffithii; Zinc
• ISSN: 0098-8472; 1873-7307
• DOI: 10.1016/j.envexpbot.2009.02.014

In this study, zinc (Zn) and cadmium (Cd) tolerance, accumulation and distribution was conducted in Potentilla griffithii H., which has been identified as a new Zn hyperaccumulator found in China. Plants were grown hydroponically with different levels of Zn 2+ (20, 40, 80 and 160 mg L −1) and Cd 2+ (5, 10, 20 and 40 mg L −1) for 60 days. All plants grew healthy and attained more biomass than the control, except 40 mg L −1 Cd treatment. Zn or Cd concentration in plants increased steadily with the increasing addition of Zn or Cd in solution. The maximum metal concentrations in roots, petioles and leaves were 14,060, 19,600 and 11,400 mg kg −1 Zn dry weight (DW) at 160 mg L −1 Zn treatment, and 9098, 3077 and 852 mg kg −1 Cd DW at 40 mg L −1 Cd treatment, respectively. These results suggest that P. griffithii has a high ability to tolerate and accumulate Cd and Zn, and it can be considered not only as Zn but also as a potential cadmium hyperaccumulator. Light microscope (LM) with histochemical method, scanning electron microscope combined with energy dispersive spectrometry (SEM-EDS) and transmission electron microscope (TEM) were used to determine the distribution of Zn and Cd in P. griffithii at tissue and cellular levels. In roots, SEM-EDS confirmed that the highest Zn concentration was found in xylem parenchyma cells and epidermal cells, while for Cd, a gradient was observed with the highest Cd concentration in rhizodermal and cortex cells, followed by central cylinder. LM results showed that Zn and Cd distributed mainly along the walls of epidermis, cortex, endodermis and some xylem parenchyma. In leaves, Zn and Cd shared the similar distribution pattern, and both were mostly accumulated in epidermis and bundle sheath. However, in leaves of 40 mg L −1 Cd treatment, which caused the phytotoxicity, Cd was also found in the mesophyll cells. The major storage site for Zn and Cd in leaves of P. griffithii was vacuoles, to a lesser extent cell wall or cytosol. The present study demonstrates that the predominant sequestration of Zn and Cd in cell walls of roots and in vacuoles of epidermis and bundle sheath of leaves may play a major role in strong tolerance and hyperaccumulation of Zn and Cd in P. griffithii.