FIRST REPORT IN ITALY OF A TOMATO RESISTANCE-BREAKING STRAIN OF TOMATO SPOTTED WILT VIRUS FROM CHRYSANTHEMUM

In 2007, samples were received from near Bari (Apulia, Southern Italy) of greenhouse-grown chrysanthemums (Den-dranthema grandiflora cvs. Boris Becker, Delianne, Eleonora, Euro, Il Mondo, Paso Doble Pink, Reagan, Taormina, Zem-bla and Snowdon) all of which showed distinct chlorotic spots on the top...

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Bibliographic Details
Published in:Journal of plant pathology 2008-03, Vol.90 (1), p.149-149
Main Authors: Bubici, G., Finetti-Sialer, M.M., Mascia, T., Amenduni, M., Cirulli, M., Gallitelli, D.
Format: Article
Language:English
Subjects:
Chrysanthemum
Chrysanthemum stem necrosis virus
Disease Note
Impatiens necrotic spot virus
Lycopersicon esculentum
Necrosis
Phytopathology
Tomato spotted wilt virus
Tospovirus
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Summary:In 2007, samples were received from near Bari (Apulia, Southern Italy) of greenhouse-grown chrysanthemums (Den-dranthema grandiflora cvs. Boris Becker, Delianne, Eleonora, Euro, Il Mondo, Paso Doble Pink, Reagan, Taormina, Zem-bla and Snowdon) all of which showed distinct chlorotic spots on the top leaves, followed by wilting and necrosis of the leaves and stems. In cvs Delianne and Snowdon, petioles and flowerheads were also necrotic. Tomato spotted wilt virus (TSWV) but not Impatiens necrotic spot virus was detected in all samples tested by dot blot hybridization with digoxigenin-labelled riboprobes (Finetti-Sialer and Gallitel-li, 2000). No amplicons were obtained in RT-PCR tests for the presence of Chrysanthemum stem necrosis virus using primers corresponding to positions 69-90 and 827-849 of RNA-M (accession No. AF213675). Three isolates obtained from cv. Snowdon and the wild-type strain p105 of TSWV were mechanically inoculated onto 4 plants (at 2-3 true leaf stage) of the F sub(1) hybrid tomato cv. Diaz, carrying the Sw5 resistance gene to TSWV, and the susceptible cv. UC82. The three Snowdon isolates, but not strain p105, overcame the resistance and induced systemic necrosis. UC82 plants became systemically infected by all 4 isolates. The results show that the Snowdon isolates are resistance-breaking. Moreover, a 670 bp DNA copy of part of the NS sub(M) gene of each Snowdon isolate yielded a MaeI restriction pattern like that of resistance-breaking strains of TSWV A-type (Finetti-Sialer et al., 2002; Ciuffo et al., 2005). This is the first report of RB strains of TSWV in chrysanthemum in Italy.
ISSN:1125-4653
2239-7264