SURVEY OF GRAPEVINE VIRUSES IN CHILE

Grapevines from six Chilean regions were surveyed for virus diseases and tested for the presence of the most important viruses. ELISA testing of 2535 samples and confirmatory RT-PCR of some ELISA-negative samples from symptomatic and symptomless vines gave the following infection rates: 6.36% for Gr...

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Bibliographic Details
Published in:Journal of plant pathology 2008-03, Vol.90 (1), p.125-130
Main Authors: Fiore, N., Prodan, S., Montealegre, J., Aballay, E., Pino, A.M., Zamorano, A.
Format: Article
Language:English
Subjects:
Arabis mosaic virus
Grapevine fanleaf virus
Grapevine fleck virus
Grapevine virus A
Grapevine virus B
Infections
Mosaic viruses
Phytopathology
Plant diseases
Plants
Reverse transcriptase polymerase chain reaction
Rootstocks
Satellite viruses
Short Communication
Strawberry latent ringspot virus
Tomato ringspot virus
Vineyards
Viruses
Vitaceae
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Summary:Grapevines from six Chilean regions were surveyed for virus diseases and tested for the presence of the most important viruses. ELISA testing of 2535 samples and confirmatory RT-PCR of some ELISA-negative samples from symptomatic and symptomless vines gave the following infection rates: 6.36% for Grapevine fanleaf virus (GFLV); 4.67% for Grapevine leafroll associated virus 1 (GLRaV-1); 16.05% for Grapevine leafroll associated virus 2 (GLRaV-2); 6.41% for Grapevine leafroll associated virus 3 (GLRaV-3); 0.26% for Grapevine leafroll associated virus 7 (GLRaV-7); 14.99% for Grapevine fleck virus (GFkV); 5.57% for Grapevine virus A (GVA) and 0.78% for Grapevine virus B (GVB). Strawberry latent ringspot virus (SLRSV) Tomato ringspot virus (ToRSV) and Arabis mosaic virus (ArMV) were not detected. Overall infection in the surveyed Chilean grapevines, considering ELISA and RT-PCR, was 32.35%. Virus infection ratio obtained from ELISA analysis in the six regions, varied between 21.19% (Región Metropolitana) and 74.26% (Coquimbo). RT-PCR was used for detection of the Red Globe strain of GLRaV-2 and Grapevine rupestris stem pitting associated virus (GRSPaV), and to confirm and extend ELISA results. GVB, GFkV, GRSPaV, GLRaV-2 RG and GLRaV-7 are new records for Chile.
ISSN:1125-4653
2239-7264