Evaluation of enterovirus recovery in surface water by different adsorption and elution procedures

The performance of six concentration method combinations and two quantitative analysis techniques were evaluated in terms of enterovirus recovery efficiencies by adjusting the pH and salt concentration of water samples. Of the six concentration method combinations, adsorption on nitrocellulose membr...

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Bibliographic Details
Published in:Chemosphere (Oxford) 2007, Vol.66 (5), p.964-969
Main Authors: Hsu, Bing-Mu, Chen, Chien-Hsien, Kung, Chien-Min, Wan, Min-Tao, Shen, Shu-Min
Format: Article
Language:English
Subjects:
Adsorption
Analysis methods
Applied sciences
Collodion - chemistry
Drinking water and swimming-pool water. Desalination
Electrochemistry
Enterovirus
Enterovirus - genetics
Enterovirus - isolation & purification
Enteroviruses
Environmental Monitoring
Exact sciences and technology
Filtration
Hydrogen-Ion Concentration
Micropore Filters
MPN-RT-PCR
Natural water pollution
Pollution
Real-time RT-PCR
Reverse Transcriptase Polymerase Chain Reaction
RNA, Viral - analysis
Water Microbiology
Water Pollutants - isolation & purification
Water treatment and pollution
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Summary:The performance of six concentration method combinations and two quantitative analysis techniques were evaluated in terms of enterovirus recovery efficiencies by adjusting the pH and salt concentration of water samples. Of the six concentration method combinations, adsorption on nitrocellulose membranes followed by an acid rinse elution consistently gave the highest recovery efficiencies. In theory, an electropositive membrane should be the most appropriate technique for adsorption of electronegative viruses in pure water. However, it displayed the greatest loss in natural waters. For adsorption and elution procedures, both the electronegative membrane, accompanied by an acid rinse step, and the electropositive membrane, accompanied by a glycine rinse step, provided higher recovery efficiencies. MPN-RT-PCR, a statistically quantitative analysis method, performed more efficiently, in economic terms, but had a similar enterovirus recovery trend to real-time RT-PCR, which is the authoritative quantitative analysis method for nucleic acid.
ISSN:0045-6535
1879-1298
DOI:10.1016/j.chemosphere.2006.06.054