NDR1/HIN1-like genes in Glycine max with potential roles in defense against the Soybean Cyst Nematode, Heterodera glycines

NDR1/HIN1-like (NHL) genes constitute a large gene family in plants that encode putative membrane associated proteins with potential functions in plant responses to pathogens downstream of initial signal recognition. Certain members of this gene family in Arabidopsis have been shown to be induced in...

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Bibliographic Details
Published in:Phytopathology 2009-06, Vol.99 (6), p.S147-S147
Main Authors: Yeckel, G J, Ithal, N, Nettleton, D, Recknor, J, Nguyen, H, Wu, X, Liu, X, Mitchum, M
Format: Article
Language:English
Subjects:
Arabidopsis
Glycine max
Heterodera glycines
Nematoda
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Summary:NDR1/HIN1-like (NHL) genes constitute a large gene family in plants that encode putative membrane associated proteins with potential functions in plant responses to pathogens downstream of initial signal recognition. Certain members of this gene family in Arabidopsis have been shown to be induced in response to avirulent bacteria, expression is suppressed by virulent bacteria, and overexpression correlates with increased resistance. However, the molecular function of NHL proteins and how they may be contributing to plant defense against pathogens remains unknown. Several soybean (Glycine max) genes showing sequence homology with Arabidopsis NHL genes were identified to be differentially expressed in microarray studies comparing the gene expression profiles of laser-microdissected feeding cells (syncytia) induced by the soybean cyst nematode (SCN; Heterodera glycines) from resistant and susceptible soybean lines. NHL promoter-GUS fusions confirmed nematode-inducible expression of these genes specifically within developing syncytia. Quantitative real-time PCR determined that the timing and level of expression of these NHL genes in response to SCN infection is faster and higher in the resistant line. Moreover, NHL transcript levels are suppressed by a virulent SCN population. We are currently assessing soybean lines overexpressing NHL genes for increased resistance to SCN.
ISSN:0031-949X