Production, characterization and antioxidant activities in vitro of exopolysaccharides from endophytic bacterium Paenibacillus polymyxa EJS-3

• Published in: Carbohydrate polymers 2009-09, Vol.78 (2), p.275-281
• Main Authors: Liu, Jun, Luo, Jianguang, Ye, Hong, Sun, Yi, Lu, Zhaoxin, Zeng, Xiaoxiong
• Format: Article
• Language: English
• Subjects: Antioxidant activity; Applied sciences; Bacteria; Bioconversions. Hemisynthesis; Biological and medical sciences; Biotechnology; Culture conditions; Endophytic bacterium; Exact sciences and technology; Exopolysaccharides; Fundamental and applied biological sciences. Psychology; General and cellular metabolism. Vitamins; Medical sciences; Methods. Procedures. Technologies; Natural polymers; Paenibacillus polymyxa; Pharmacology. Drug treatments; Physicochemistry of polymers; Starch and polysaccharides
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2009.03.046

The production, characterization and antioxidant activities in vitro of exopolysaccharides (EPS) from endophytic bacterium Paenibacillus polymyxa EJS-3 were investigated. For EPS production, the preferable culture conditions were 24 °C and pH 8 for 60 h with sucrose and yeast extract as the carbon and nitrogen sources, respectively. Notably, sucrose concentration was the prominent factor, and the maximum yield of EPS (22.82 g/L) was obtained at a sucrose concentration of 160 g/L. The crude EPS was purified by chromatography of DEAE-52 and Sephadex G-100, affording EPS-1 and EPS-2 with molecular weights of 1.22 × 10 6 and 8.69 × 10 5 Da, respectively. They were composed of mannose, fructose and glucose in a molar ratio of 2.59:29.83:1 and 4.23:36.59:1, respectively. In addition, both crude and purified EPS showed strong scavenging activities on superoxide and hydroxyl radicals, and their antioxidant activities decreased in the order of crude EPS > EPS-2 > EPS-1.