Mechanism of interaction of tumor necrosis factor (TNF-a) of macroorganism with Salmonella Enterica cells (Ser. Typhimurium)

• Published in: Molecular genetics, microbiology and virology microbiology and virology, 2008-12, Vol.23 (4), p.178-183
• Main Authors: Romanova, Yu. M., Tomova, A. S., Shingarova, L. N., Lunin, V. G., Karyagina, A. S., Lupu, I. P., Gintsburg, A. L.
• Format: Article
• Language: English
• Subjects: Biomedical and Life Sciences; Experimental Papers; Life Sciences; Microbiology; Molecular Medicine; Salmonella enterica
• ISSN: 0891-4168; 1934-841X
• DOI: 10.3103/S0891416808040046

The goal of this work was to elucidate the mechanism of the direct interaction of bacteria cells with tumor necrosis factor (TNF-α, cytokine). It was shown previously that this interaction facilitated the activation of the bacterial growth and recultivation of noncultivated forms in vitro and in vivo. Mice deficient in genes that encode eukaryotic TNF-α receptors were infected with salmonella cells. When exogenous TNF-α was introduced into the suspension of the infected cells, the infection process (seeding of bacteria from spleens) started one day earlier in both the knockout and control mice, in comparison with that in the absence of cytokine. Bacteria are therefore able to interact with cytokine, even in the absence of eukaryotic receptors. The specificity of the bacteria-cytokine interaction was demonstrated and bacterial protein EF-Tu, which mediates the direct interaction of bacteria with cytokine, was identified using the method of immobilization of the recombinant protein TNF-α-spacer-CBD on cellulose.