Injectable platelet rich fibrin: cell content, morphological, and protein characterization

• Published in: Clinical oral investigations 2019-03, Vol.23 (3), p.1309-1318
• Main Authors: Varela, Hugo Almeida, Souza, Júlio C. M., Nascimento, Rubens M., Araújo, Raimundo F., Vasconcelos, Roseane C., Cavalcante, Rômulo S., Guedes, Paulo M., Araújo, Aurigena A.
• Format: Article
• Language: English
• Subjects: Adult; Biological activity; Biomaterials; Blood clots; Blood coagulation; Blood platelets; Blood Platelets - cytology; Collagen; Collagen (type I); Collagen Type I - chemistry; Dentistry; Embedding; Enzyme-linked immunosorbent assay; Fibrin; Fibrin - chemistry; Gene expression; Growth factors; Humans; Immunohistochemistry; Interleukin 10; Interleukin-10 - chemistry; Leukocytes; Leukocytes - cytology; Lymphocytes; Male; Medicine; Morphology; Original Article; Osteocalcin; Osteocalcin - chemistry; Periodontics; Peripheral blood; Platelet-derived growth factor; Platelet-Rich Fibrin - chemistry; Platelet-Rich Fibrin - cytology; Platelets; Polymerase chain reaction; RNA-directed DNA polymerase; Scanning electron microscopy; Transforming Growth Factor beta1 - chemistry; Transforming growth factor-b; Vascular endothelial growth factor; Vascular Endothelial Growth Factor A - chemistry
• ISSN: 1432-6981; 1436-3771
• DOI: 10.1007/s00784-018-2555-2

Objectives The aim of the present study was to evaluate the blood cell content, morphological aspects, gene expression of type I collagen, and release of growth factors on an injectable platelet rich fibrin (i-PRF). Materials and methods Blood samples were collected from 15 volunteers to prepare i-PRF samples. Peripheral blood was used as a control group. Blood clot and i-PRF samples were cultured for 10 days. The supernatant of the samples was collected for ELISA immunoassay quantification of PDGF and VEGF growth factors over periods of 1, 8, 24, 72, and 240 h. I-PRF and blood clot samples were biologically characterized using histological and immunohistochemistry analysis for IL-10, osteocalcin, and TGF-β. Scanning electron microscopy (SEM) was used to inspect the fibrin network and distribution of blood platelets and leukocytes. Reverse transcriptase polymerase chain reaction (RT-PCR) method was used to evaluate gene expression for type I collagen. Results A higher concentration of platelets and lymphocytes was recorded in i-PRF than in peripheral blood ( p