Amperometric Biosensors for Sensing of Hydrogen Peroxide Based on Electron Transfer between Horseradish Peroxidase and Ferrocene as a Mediator

A non-conducting polymer film of 3-aminophenol, in which horseradish peroxidase was immobilized, has been prepared on a carbon paste electrode containing both ferrocene as a mediator and horseradish peroxidase by the electrochemical polymerization. The response currents of this hydrogen peroxide sen...

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Bibliographic Details
Published in:Analytical Sciences 2000, Vol.16(6), pp.609-613
Main Authors: NAKABAYASHI, Yasuo, YOSHIKAWA, Hajime
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biosensors
Biotechnology
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Various methods and equipments
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Summary:A non-conducting polymer film of 3-aminophenol, in which horseradish peroxidase was immobilized, has been prepared on a carbon paste electrode containing both ferrocene as a mediator and horseradish peroxidase by the electrochemical polymerization. The response currents of this hydrogen peroxide sensor linearly increased with increasing the concentration of hydrogen peroxide of 0.6 to 20 μmol dm-3; the straight line had a slope equal to 10.1 mA mol-1 dm3 (r= 0.999). This hydrogen peroxide sensor may be available for the fabrication of other biosensors, e.g. glucose and cholesterol sensors, which are not influenced by easily oxidizable species, L -ascorbic acid and uric acid.
ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.16.609