Lupeol against high-glucose-induced apoptosis via enhancing the anti-oxidative stress in rabbit nucleus pulposus cells

Purpose This study aimed to investigate the potential mechanism and value of lupeol in inhibiting high-glucose-induced apoptosis in rabbit nucleus pulposus cells (NPCs). Methods NPCs were divided into four groups: control (CON), high glucose (HG), LUP, and HG + LUP. Viability, reactive oxygen specie...

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Bibliographic Details
Published in:European spine journal 2018-10, Vol.27 (10), p.2609-2620
Main Authors: Guo, Ming-Bo, Wang, De-Chun, Liu, Hai-Fei, Chen, Long-Wei, Wei, Jian-Wei, Lin, Yong, Xue, Hui
Format: Article
Language:English
Subjects:
Apoptosis
BAX protein
Bcl-2 protein
Caspase
Caspase-9
Cytochrome
Cytochrome c
Degeneration
Gene expression
Glucose
Intervertebral discs
Medicine
Medicine & Public Health
Mitochondria
Neurosurgery
Nuclei
Nucleus pulposus
Original Article
Oxidative stress
Polymerase chain reaction
Reactive oxygen species
Reverse transcription
Surgical Orthopedics
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Summary:Purpose This study aimed to investigate the potential mechanism and value of lupeol in inhibiting high-glucose-induced apoptosis in rabbit nucleus pulposus cells (NPCs). Methods NPCs were divided into four groups: control (CON), high glucose (HG), LUP, and HG + LUP. Viability, reactive oxygen species (ROS) levels, and apoptosis were examined in NPCs. The protein expression levels of Bax, Bcl-2, cytochrome C, and caspase 9/3 were measured using reverse transcription–polymerase chain reaction and Western blot assay. Results The apoptotic rate and total ROS level of the HG group significantly increased compared with the CON group ( P  
ISSN:0940-6719
1432-0932
DOI:10.1007/s00586-018-5687-9