Dynamic expression of vasotocin and isotocin receptor genes in the marbled eel (Anguilla marmorata) following osmotic challenges

• Published in: Gene 2018-11, Vol.677, p.49-56
• Main Authors: Cao, Quanquan, Liang, Fenfei, Wang, Dan, Zhang, Xinyu, Lorin-Nebel, Catherine, Gu, Jie, Yin, Shaowu
• Format: Article
• Language: English
• Subjects: Anguilla - genetics; Animals; AVTR; Eel; Fresh Water; Gills - metabolism; ITR; Osmoregulation; Osmoregulation - genetics; Oxytocin - analogs & derivatives; Oxytocin - genetics; Receptors, Vasopressin - genetics; RNA, Messenger - genetics; Salinity; Seawater; Vasotocin - genetics; Water-Electrolyte Balance - genetics
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/j.gene.2018.07.021

To examine the physiological roles of arginine vasotocin receptor (AVTR) and isotocin receptor (ITR) in osmoregulation of a euryhaline teleost, the marbled eel (Anguilla marmorata), three different genes coding for AVTRV1a2, AVTRV2 and ITR were cloned by screening an A. marmorata cDNA library. These receptors were expressed differentially and ubiquitously in the eight tissues we examined. The changes in mRNA expression levels of AVTRV1a2, AVTRV2, and ITR were assessed in a time-course study following salinity transfer from fresh water (FW, 0‰) to fresh water (FW, 0‰), brackish water (BW, 10‰) or saline water (SW, 25‰). When eels were transferred to BW, mRNA levels underwent an adaptive period, from 0 to 24 h, and a chronic regulatory period, starting at 24 h after transfer. In the adaptive period, the relative mRNA expression of AVTRV1a2, AVTRV2, and ITR increased in BW. But after this adaptive period, the mRNA levels of the three genes were significantly decreased compared to FW (control group, 0 h). The mRNA expression levels of AVTRV1a2, AVTRV2 and ITR were low in SW. The protein level of AVTRV1a2, a key protein in the brain, was also investigated and found to be consistent with mRNA results. Our results indicated that the nonapeptide receptor system may play a role in the acute stress response induced by hyper-osmotic challenge in marbled eels. •Full-length cDNAs encoding AVTRV1a2, AVTRV2 and ITR were cloned in A. marmorata.•These receptors were expressed ubiquitously in eight examined tissues.•The mRNA and protein temporal expressions were stated in salinity acclimation.