Biological markers in cerebrospinal fluid (CSF) and evaluation of in vitro effect of CSF on PC12 cell line viability in Alzheimer's disease

• Published in: Cell biochemistry and function 2009-08, Vol.27 (6), p.395-401
• Main Authors: Yaka, Erdem, Egrilmez, Mehtap Yuksel, Keskinoglu, Pembe, Cavdar, Zahide, Genc, Sermin, Genc, Kursad, İyilikci, Leyla, Yener, Görsev G.
• Format: Article
• Language: English
• Subjects: Aged; Alzheimer Disease - cerebrospinal fluid; Alzheimer Disease - diagnosis; Alzheimer's disease; Amyloid beta-Peptides - cerebrospinal fluid; Amyloid beta-Peptides - toxicity; Animals; Biomarkers - cerebrospinal fluid; Cell Survival; Chi-Square Distribution; CSF markers; cytotoxicity; Cytotoxins - cerebrospinal fluid; Cytotoxins - toxicity; Dementia; Female; Humans; Male; Middle Aged; PC12 cell line; PC12 Cells; Peptide Fragments - cerebrospinal fluid; Peptide Fragments - toxicity; Rats; ROC Curve; Statistics, Nonparametric; tau; tau Proteins - cerebrospinal fluid; tau Proteins - toxicity
• ISSN: 0263-6484; 1099-0844
• DOI: 10.1002/cbf.1588

The definite diagnosis of Alzheimer's disease (AD) is based on post mortem pathological examination. To date, there is no laboratory test that can discriminate AD patients from healthy individuals. In the perspective of recent knowledge, there are three cerebrospinal fluid (CSF) markers which have the highest sensitivity and specificity: Aβ1–40, Aβ1–42, and p‐tau. In the present study, 15 ‘Probable Alzheimer's Disease’ (PAD) patients and 15 control subjects were included. PAD patients were selected from the patients of Dokuz Eylül University Neurology Department Dementia outpatient clinic and control subjects were selected from the patients who were undergone epidural anesthesia because of any surgical operation. The concentrations of Ab1–40, Ab1–42, and p‐tau in CSF were quantified by using ELISA. Also, the effects of ‘PAD patients’ CSF on the survival of PC12 cell line were assessed. There was a significant decrease of Ab1–40 and increase of p‐tau in patients with AD when compared with controls. Ab1–42 concentration was not significantly different between groups. There was a positive corelation between duration of the disease and CSF of p‐tau concentration in patients with AD. There was no significant difference in cell line viability values between groups. Copyright © 2009 John Wiley & Sons, Ltd.