Rice PPS1 encodes a DYW motif-containing pentatricopeptide repeat protein required for five consecutive RNA-editing sites of nad3 in mitochondria

• Published in: The New phytologist 2018-11, Vol.220 (3), p.878-892
• Main Authors: Xiao, Haijun, Zhang, Qiannan, Qin, Xiaojian, Xu, Yanghong, Ni, Chenzi, Huang, Jishuai, Zhu, Linlin, Zhong, Feiya, Liu, Wei, Yao, Guoxin, Zhu, Yingguo, Hu, Jun
• Format: Article
• Language: English
• Subjects: Analytical methods; Cytoplasmic organelles; DNA probes; Editing; Electron transport; Electron transport chain; Electrophoresis; Electrophoretic mobility; Gene expression; Leucine; Mitochondria; Morphology; nad3; Nucleic acids; Organelles; pentatricopeptide repeat; Plants (botany); Pollen; pollen partial sterility; Proline; Proteins; Regulators; Ribonucleic acid; rice; RNA; RNA editing; RNA-mediated interference; Sterility; Transcription
• ISSN: 0028-646X; 1469-8137
• DOI: 10.1111/nph.15347

The pentatricopeptide repeat (PPR) protein family is a large family characterized by tandem arrays of a degenerate 35-amino-acid motif whose members function as important regulators of organelle gene expression at the post-transcriptional level. Despite the roles of PPRs in RNA editing in organelles, their editing activities and the underlying mechanism remain obscure. Here, we show that a novel DYW motif-containing PPR protein, PPS1, is associated with five conserved RNA-editing sites of nad3 located in close proximity to each other in mitochondria, all of which involve conversion from proline to leucine in rice. Both pps1 RNAi and heterozygous plants are characterized by delayed development and partial pollen sterility at vegetative stages and reproductive stage. RNA electrophoresis mobility shift assays (REMSAs) and reciprocal competition assays using different versions of nad3 probes confirm that PPS1 can bind to cis-elements near the five affected sites, which is distinct from the existing mode of PPR-RNA binding because of the continuity of the editing sites. Loss of editing at nad3 in pps1 reduces the activity of several complexes in the mitochondrial electron transport chain and affects mitochondrial morphology. Taken together, our results indicate that PPS1 is required for specific editing sites in nad3 in rice.