Isolation and Identification of Lactic Acid Bacteria Isolated from a Traditional Jeotgal Product in Korea

Seventeen lactic acid bacterial strains (LAB) were isolated using MRS agar medium from Jeotgal, a Korean fermented seafood, purchased at the Jukdo market of Pohang. To identify the strains isolated, they were tested by examining their cell morphologies, gram-staining, catalase activity, arginine hyd...

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Bibliographic Details
Published in:Ocean science journal 2006-06, Vol.41 (2), p.113-119
Main Authors: Cho, Gyu Sung, Do, Hyung Ki
Format: Article
Language:English
Subjects:
Enterococcus
Lactobacillus
Leuconostoc
Leuconostoc mesenteroides
Marine
Pediococcus
Streptococcus
Streptococcus salivarius
Weissella
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Summary:Seventeen lactic acid bacterial strains (LAB) were isolated using MRS agar medium from Jeotgal, a Korean fermented seafood, purchased at the Jukdo market of Pohang. To identify the strains isolated, they were tested by examining their cell morphologies, gram-staining, catalase activity, arginine hydrolase activity, D-L lactate form and carbohydrate fermentation. According to the phenotypic characteristics, three strains were tentatively identified as Lactobacillus spp., ten were Enterococcus spp. (or Streptococcus spp., or Pediococcus spp.) and the rest were Leuconostoc spp. (or Weissella spp.). Five strains among 17 were chosen by preliminary bacteriocin activity test. Four bacterial strains which inhibited both indicator microorganisms were identified by 16S rRNA sequencing. The results are as follows; Leuconostoc mesenteroides (HK 4), Leuconostoc mesenteroides (HK 5), Leuconostoc mesenteroides(HK 11), Streptococcus salivarius(HK 8). In order to check LAB which are showing a high survival rate in gut, we investigated three strains inhibiting both indicator microorganisms in artificial gastric acid and bile juice -all except HK8. The three strains mentioned above grew in extreme low acid conditions.
ISSN:1738-5261
DOI:10.1007/BF03022416