Influence of explanting season on in vitro multiplication of the medicinal herb, Tridax procumbens L

A protocol for complete regeneration of Tridax procumbens, a medicinally important plant, was accomplished through in vitro culturing. From nodal segments explants, high percentage of bud break and multiple shoot formation was induced between July and September on MS medium supplemented with BAP (1...

Full description

Saved in:
Bibliographic Details
Published in:African journal of biotechnology 2009-07, Vol.8 (14), p.3239-3243
Main Authors: Malik, C P, Wadhwani, C
Format: Article
Language:English
Subjects:
Tridax procumbens
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page 3243
container_issue 14
container_start_page 3239
container_title African journal of biotechnology
container_volume 8
creator Malik, C P
Wadhwani, C
description A protocol for complete regeneration of Tridax procumbens, a medicinally important plant, was accomplished through in vitro culturing. From nodal segments explants, high percentage of bud break and multiple shoot formation was induced between July and September on MS medium supplemented with BAP (1 mg L super(-1)). Rooting of the excised shoots from secondary cultures was excellent on half-strength MS medium having 1 mg L super(-1) IBA. Micropropagated plants when transplanted on garden soil flowered after 6 - 8 weeks. Explants sampled during December had low percentage of bud break and less shoot number per explant. Epicuticular wax (EW) and cuticular transpiration varied in the leaves collected during July and December; while under in vitro conditions, no marked differences were observed in regards to the 2 parameters. The epicuticular contents correlated with the rate of cuticular transpiration. The adaptive significance of EW during December is discussed.
format article
fullrecord <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_miscellaneous_20721334</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>20721334</sourcerecordid><originalsourceid>FETCH-LOGICAL-p186t-5ec62173056f63f142299955b79fe3123f89c2e62c05edfce8b35e094b0168963</originalsourceid><addsrcrecordid>eNpNj01LxDAYhIMouK77H96TJwv5aNLmKIsfCwUv63lJ0ze7kTStTSr7863oYWFgBgZmeK7Iiqm6LKRg8voi35K7lD4p5YKXdEW6XXRhxmgRBgd4HoOJ2ccjJDRpiLDIR_j2eRqgn0P2Y_DWZP9bOcgnhB47b300AU44tY-wn3xnzjBOg537FmOC5p7cOBMSbv59TT5envfbt6J5f91tn5piZLXKhUSrOKsElcop4VjJudZayrbSDgXjwtXaclTcUomds1i3QiLVZUsXPK3Emjz87S7nXzOmfOh9shgWJhzmdOC04kyIUvwAdc9UVg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20721334</pqid></control><display><type>article</type><title>Influence of explanting season on in vitro multiplication of the medicinal herb, Tridax procumbens L</title><source>Free Full-Text Journals in Chemistry</source><creator>Malik, C P ; Wadhwani, C</creator><creatorcontrib>Malik, C P ; Wadhwani, C</creatorcontrib><description>A protocol for complete regeneration of Tridax procumbens, a medicinally important plant, was accomplished through in vitro culturing. From nodal segments explants, high percentage of bud break and multiple shoot formation was induced between July and September on MS medium supplemented with BAP (1 mg L super(-1)). Rooting of the excised shoots from secondary cultures was excellent on half-strength MS medium having 1 mg L super(-1) IBA. Micropropagated plants when transplanted on garden soil flowered after 6 - 8 weeks. Explants sampled during December had low percentage of bud break and less shoot number per explant. Epicuticular wax (EW) and cuticular transpiration varied in the leaves collected during July and December; while under in vitro conditions, no marked differences were observed in regards to the 2 parameters. The epicuticular contents correlated with the rate of cuticular transpiration. The adaptive significance of EW during December is discussed.</description><identifier>ISSN: 1684-5315</identifier><identifier>EISSN: 1684-5315</identifier><language>eng</language><subject>Tridax procumbens</subject><ispartof>African journal of biotechnology, 2009-07, Vol.8 (14), p.3239-3243</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids></links><search><creatorcontrib>Malik, C P</creatorcontrib><creatorcontrib>Wadhwani, C</creatorcontrib><title>Influence of explanting season on in vitro multiplication of the medicinal herb, Tridax procumbens L</title><title>African journal of biotechnology</title><description>A protocol for complete regeneration of Tridax procumbens, a medicinally important plant, was accomplished through in vitro culturing. From nodal segments explants, high percentage of bud break and multiple shoot formation was induced between July and September on MS medium supplemented with BAP (1 mg L super(-1)). Rooting of the excised shoots from secondary cultures was excellent on half-strength MS medium having 1 mg L super(-1) IBA. Micropropagated plants when transplanted on garden soil flowered after 6 - 8 weeks. Explants sampled during December had low percentage of bud break and less shoot number per explant. Epicuticular wax (EW) and cuticular transpiration varied in the leaves collected during July and December; while under in vitro conditions, no marked differences were observed in regards to the 2 parameters. The epicuticular contents correlated with the rate of cuticular transpiration. The adaptive significance of EW during December is discussed.</description><subject>Tridax procumbens</subject><issn>1684-5315</issn><issn>1684-5315</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNpNj01LxDAYhIMouK77H96TJwv5aNLmKIsfCwUv63lJ0ze7kTStTSr7863oYWFgBgZmeK7Iiqm6LKRg8voi35K7lD4p5YKXdEW6XXRhxmgRBgd4HoOJ2ccjJDRpiLDIR_j2eRqgn0P2Y_DWZP9bOcgnhB47b300AU44tY-wn3xnzjBOg537FmOC5p7cOBMSbv59TT5envfbt6J5f91tn5piZLXKhUSrOKsElcop4VjJudZayrbSDgXjwtXaclTcUomds1i3QiLVZUsXPK3Emjz87S7nXzOmfOh9shgWJhzmdOC04kyIUvwAdc9UVg</recordid><startdate>20090720</startdate><enddate>20090720</enddate><creator>Malik, C P</creator><creator>Wadhwani, C</creator><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20090720</creationdate><title>Influence of explanting season on in vitro multiplication of the medicinal herb, Tridax procumbens L</title><author>Malik, C P ; Wadhwani, C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p186t-5ec62173056f63f142299955b79fe3123f89c2e62c05edfce8b35e094b0168963</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Tridax procumbens</topic><toplevel>online_resources</toplevel><creatorcontrib>Malik, C P</creatorcontrib><creatorcontrib>Wadhwani, C</creatorcontrib><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>African journal of biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Malik, C P</au><au>Wadhwani, C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of explanting season on in vitro multiplication of the medicinal herb, Tridax procumbens L</atitle><jtitle>African journal of biotechnology</jtitle><date>2009-07-20</date><risdate>2009</risdate><volume>8</volume><issue>14</issue><spage>3239</spage><epage>3243</epage><pages>3239-3243</pages><issn>1684-5315</issn><eissn>1684-5315</eissn><abstract>A protocol for complete regeneration of Tridax procumbens, a medicinally important plant, was accomplished through in vitro culturing. From nodal segments explants, high percentage of bud break and multiple shoot formation was induced between July and September on MS medium supplemented with BAP (1 mg L super(-1)). Rooting of the excised shoots from secondary cultures was excellent on half-strength MS medium having 1 mg L super(-1) IBA. Micropropagated plants when transplanted on garden soil flowered after 6 - 8 weeks. Explants sampled during December had low percentage of bud break and less shoot number per explant. Epicuticular wax (EW) and cuticular transpiration varied in the leaves collected during July and December; while under in vitro conditions, no marked differences were observed in regards to the 2 parameters. The epicuticular contents correlated with the rate of cuticular transpiration. The adaptive significance of EW during December is discussed.</abstract><tpages>5</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1684-5315
ispartof African journal of biotechnology, 2009-07, Vol.8 (14), p.3239-3243
issn 1684-5315
1684-5315
language eng
recordid cdi_proquest_miscellaneous_20721334
source Free Full-Text Journals in Chemistry
subjects Tridax procumbens
title Influence of explanting season on in vitro multiplication of the medicinal herb, Tridax procumbens L
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T21%3A46%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Influence%20of%20explanting%20season%20on%20in%20vitro%20multiplication%20of%20the%20medicinal%20herb,%20Tridax%20procumbens%20L&rft.jtitle=African%20journal%20of%20biotechnology&rft.au=Malik,%20C%20P&rft.date=2009-07-20&rft.volume=8&rft.issue=14&rft.spage=3239&rft.epage=3243&rft.pages=3239-3243&rft.issn=1684-5315&rft.eissn=1684-5315&rft_id=info:doi/&rft_dat=%3Cproquest%3E20721334%3C/proquest%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-p186t-5ec62173056f63f142299955b79fe3123f89c2e62c05edfce8b35e094b0168963%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=20721334&rft_id=info:pmid/&rfr_iscdi=true