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Phycobiliprotein fluorescence of Nostoc punctiforme changes during the life cycle and chromatic adaptation: characterization by spectral confocal laser scanning microscopy and spectral unmixing

Many cyanobacteria are highly adaptable to light quality, and many species undergo a complex life cycle. In this study we show that adaptive changes in the photosynthetic apparatus of cyanobacteria are not only caused by environmental, but also by developmental factors. Spectral confocal laser scann...

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Published in:Plant, cell and environment cell and environment, 2005-04, Vol.28 (4), p.480-491
Main Authors: Wolf, E, Schussler, A
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description Many cyanobacteria are highly adaptable to light quality, and many species undergo a complex life cycle. In this study we show that adaptive changes in the photosynthetic apparatus of cyanobacteria are not only caused by environmental, but also by developmental factors. Spectral confocal laser scanning microscopy (CLSM) was used to analyse in vivo the fluorescence spectra of the photosynthetic pigments chlorophyll a (Chl a), allophycocyanin (APC), phycocyanin (PC) and phycoerythrin (PE) of two Nostoc punctiforme strains. Changes in pigment fluorescence emission occurred in different developmental stages. Strain 1:1-26 showed an emission maximum at 674 nm in motile hormogonia stages, whereas vegetative stages showed maxima at 658 and 575 nm. These changes were not caused by chromatic adaptation. In contrast, the second strain (1:1-26lg) showed distinct fluorescence spectra, pigment localization and clear chromatic adaptation in red light. When these properties are known, both strains can be easily distinguished by the spectral CLSM method, which also allows the localization of the pigments within single cells. To calculate the contribution of individual phycobiliproteins to the observed changes, fluorescence spectra were analysed by spectral unmixing. This allowed the mathematical estimation of fluorescence shares for the individual phycobiliproteins in different developmental stages and both before and after chromatic adaptation. It is concluded that care should be taken when characterizing cyanobacteria by differences in pigment fluorescence, because these differences are influenced not only by chromatic adaptation, but also developmental stages. Spectral CLSM offers a powerful method to study the phycobiliprotein composition in vivo.
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In this study we show that adaptive changes in the photosynthetic apparatus of cyanobacteria are not only caused by environmental, but also by developmental factors. Spectral confocal laser scanning microscopy (CLSM) was used to analyse in vivo the fluorescence spectra of the photosynthetic pigments chlorophyll a (Chl a), allophycocyanin (APC), phycocyanin (PC) and phycoerythrin (PE) of two Nostoc punctiforme strains. Changes in pigment fluorescence emission occurred in different developmental stages. Strain 1:1-26 showed an emission maximum at 674 nm in motile hormogonia stages, whereas vegetative stages showed maxima at 658 and 575 nm. These changes were not caused by chromatic adaptation. In contrast, the second strain (1:1-26lg) showed distinct fluorescence spectra, pigment localization and clear chromatic adaptation in red light. 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Spectral CLSM offers a powerful method to study the phycobiliprotein composition in vivo.</description><subject>adaptation</subject><subject>allophycocyanin</subject><subject>Biological and medical sciences</subject><subject>chlorophyll</subject><subject>confocal scanning laser microscopy</subject><subject>cyanobacteria</subject><subject>Enzymes</subject><subject>fluorescence</subject><subject>fluorescence spectra</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>hormogonia</subject><subject>in vivo measurements</subject><subject>life cycle</subject><subject>life cycle (organisms)</subject><subject>light harvesting complex</subject><subject>Metabolism</subject><subject>Nostoc</subject><subject>Nostoc punctiforme</subject><subject>photosynthesis</subject><subject>photosynthetic pigments</subject><subject>phycobiliprotein</subject><subject>phycobilisomes</subject><subject>phycocyanin</subject><subject>phycoerythrin</subject><subject>Plant physiology and development</subject><subject>plant pigments</subject><subject>spectral analysis</subject><subject>spectral confocal laser scanning microscopy</subject><issn>0140-7791</issn><issn>1365-3040</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNqNkd-KEzEUxgdRsK4-g0HQu9aTZDJ_BC-krKuw6ILudThNkzYlk4zJDNvx7XyzzbTLCl6ZmxzO_L7vZM5XFITCiubz_rCivBJLDiWsGIBYAWUtrI5PisXjh6fFAmgJy7pu6fPiRUoHgNyo20Xx52Y_qbCxzvYxDNp6YtwYok5Ke6VJMORbSENQpB-9GqwJsdNE7dHvdCLbMVq_I8NeE2dN7k_KaYJ-m4kYOhysIrjFfshV8B9mXUQ16Gh_nzpkM5HUazVEdEQFb4LKhcOkI0kKvZ_dO6tiSCr008n5kR99Z48ZeFk8M-iSfvVwXxS3ny9_rr8sr79ffV1_ul4qUVJYckTBkQneiFaXbSMEbTSvjKJVK7jhWw602SBTgJQJw_hGibquzbZEWlblhl8U786-eVG_Rp0G2dm8JefQ6zAmyaBmTV3SDL75BzyEMfr8Nsl4BXWTp2eoOUPzz6Wojeyj7TBOkoKcg5UHOecn5_zkHKw8BSuPWfr2wR_zkpyJ6JVNf_VVBXkMy9zHM3dnnZ7-21_erC_nKutfn_UGg8RdzDNufzCgHKBtGasrfg8YpMQn</recordid><startdate>200504</startdate><enddate>200504</enddate><creator>Wolf, E</creator><creator>Schussler, A</creator><general>Blackwell Science Ltd</general><general>Blackwell</general><general>Wiley Subscription Services, Inc</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7ST</scope><scope>C1K</scope><scope>SOI</scope><scope>M7N</scope></search><sort><creationdate>200504</creationdate><title>Phycobiliprotein fluorescence of Nostoc punctiforme changes during the life cycle and chromatic adaptation: characterization by spectral confocal laser scanning microscopy and spectral unmixing</title><author>Wolf, E ; Schussler, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5410-3aa53a253859e4985518e36fc16953f3d3018ba2c0a125f23bc5777fd4a1464b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>adaptation</topic><topic>allophycocyanin</topic><topic>Biological and medical sciences</topic><topic>chlorophyll</topic><topic>confocal scanning laser microscopy</topic><topic>cyanobacteria</topic><topic>Enzymes</topic><topic>fluorescence</topic><topic>fluorescence spectra</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>hormogonia</topic><topic>in vivo measurements</topic><topic>life cycle</topic><topic>life cycle (organisms)</topic><topic>light harvesting complex</topic><topic>Metabolism</topic><topic>Nostoc</topic><topic>Nostoc punctiforme</topic><topic>photosynthesis</topic><topic>photosynthetic pigments</topic><topic>phycobiliprotein</topic><topic>phycobilisomes</topic><topic>phycocyanin</topic><topic>phycoerythrin</topic><topic>Plant physiology and development</topic><topic>plant pigments</topic><topic>spectral analysis</topic><topic>spectral confocal laser scanning microscopy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wolf, E</creatorcontrib><creatorcontrib>Schussler, A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Calcium &amp; Calcified Tissue Abstracts</collection><collection>Environment Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Plant, cell and environment</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wolf, E</au><au>Schussler, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phycobiliprotein fluorescence of Nostoc punctiforme changes during the life cycle and chromatic adaptation: characterization by spectral confocal laser scanning microscopy and spectral unmixing</atitle><jtitle>Plant, cell and environment</jtitle><date>2005-04</date><risdate>2005</risdate><volume>28</volume><issue>4</issue><spage>480</spage><epage>491</epage><pages>480-491</pages><issn>0140-7791</issn><eissn>1365-3040</eissn><coden>PLCEDV</coden><abstract>Many cyanobacteria are highly adaptable to light quality, and many species undergo a complex life cycle. 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subjects adaptation
allophycocyanin
Biological and medical sciences
chlorophyll
confocal scanning laser microscopy
cyanobacteria
Enzymes
fluorescence
fluorescence spectra
Fundamental and applied biological sciences. Psychology
hormogonia
in vivo measurements
life cycle
life cycle (organisms)
light harvesting complex
Metabolism
Nostoc
Nostoc punctiforme
photosynthesis
photosynthetic pigments
phycobiliprotein
phycobilisomes
phycocyanin
phycoerythrin
Plant physiology and development
plant pigments
spectral analysis
spectral confocal laser scanning microscopy
title Phycobiliprotein fluorescence of Nostoc punctiforme changes during the life cycle and chromatic adaptation: characterization by spectral confocal laser scanning microscopy and spectral unmixing
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